| 毒性中药蟾酥质量检测方法研究 |
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| 引用本文： | 王兆基,粟晓黎,刘秋铭,林瑞超,郑秀云,冯国培.毒性中药蟾酥质量检测方法研究[J].药物分析杂志,2011(6). |
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| 作者姓名： | 王兆基 粟晓黎 刘秋铭 林瑞超 郑秀云 冯国培 |
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| 作者单位： | 香港特别行政区政府化验所;中国药品生物制品检定所;香港中文大学; |
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| 摘 要： | 目的:建立蟾蜍中多种药效及毒性成分蟾蜍毒素的定性定量检测方法。方法:采用液相色谱-质谱/质谱联用方法检测蟾酥中多种蟾蜍毒素成分。结果:建立了华蟾毒它灵、蟾蜍灵、华蟾毒配基及脂蟾毒配基的LC-MS/MS检测方法。比较了蟾酥样本的液相色谱-质谱/质谱联用分析数据。对4个不同来源中华大蟾蜍及黑眶蟾蜍的蟾酥样本进行检测,检出华蟾毒它灵、蟾蜍灵、华蟾毒配基及脂蟾毒配基的含量范围为0.05%~5.12%(w/w)。结论:液相色谱-质谱/质谱联用方法可用于蟾酥定性定量检测,并为质量评价提供参考。
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| 关 键 词： | 中药材 蟾酥 蟾蜍毒素 华蟾毒它灵 蟾蜍灵 脂蟾毒配基 华蟾毒配基 液相色谱-质谱/质谱联用 |
Study on quality control method for Venenum Bufonis |
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| WONG Siu-kay,SU Xiao-li,LAU Chau-ming,LIN Rui-chao,CHENG Sau-wan,FUNG Kwok-pui.Study on quality control method for Venenum Bufonis[J].Chinese Journal of Pharmaceutical Analysis,2011(6). |
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| Authors: | WONG Siu-kay SU Xiao-li LAU Chau-ming LIN Rui-chao CHENG Sau-wan FUNG Kwok-pui |
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| Affiliation: | WONG Siu-kay1,SU Xiao-li2,LAU Chau-ming1,LIN Rui-chao2,CHENG Sau-wan3,FUNG Kwok-pui3(1.Government Laboratory,HKSAR,Hongkong,China,2.National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050,3.The Chinese University of Hong Kong,China) |
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| Abstract: | Objective:To establish quality control method for Venenum Bufonis(Chansu) which was secretion material obtained from Bufo gargarizans Cantor or Bufo melanostictus Schneider.Chansu contains bufadienolide steriods with both therapeutic properties and toxicity.Method:Using LC-MS/MS technique to establish quality control method for Chansu by determining the bufadienolide steriods.Results:Established LC-MS/MS method was used to determine 4 bufadienolide steroids viz.cinobufotalin,bufalin,resibufagenin and cinobu... |
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| Keywords: | traditional Chinese medicine Venenum Bufonis bufadienolide steroids cinobufotalin bufalin resibufagenin cinobufagin LC-MS/MS |
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