| 毛细管GC法测定不同产地青蒿中樟脑和冰片的含量 |
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| 引用本文： | 王怀玉,彭锐,王征帆,马鹏.毛细管GC法测定不同产地青蒿中樟脑和冰片的含量[J].中国药房,2012(15):1395-1396. |
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| 作者姓名： | 王怀玉 彭锐 王征帆 马鹏 |
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| 作者单位： | 绵阳师范学院;重庆市中药研究院;渭南师范学院 |
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| 基金项目： | 国家科技支撑计划子课题(2006BAI09B02-4) |
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| 摘 要： | 目的:建立测定青蒿中樟脑和冰片含量的方法。方法:采用毛细管气相色谱法。色谱柱为HP-5石英毛细管柱(30m×0.25mm×0.25μm),检测器为火焰离子化检测器,检测器温度为280℃,进样口温度为250℃,程序升温,流速为1.2mL.min-1,分流比为20:1。结果:樟脑和冰片的检测浓度分别在1.16～46.40、1.24～49.60μg.mL-1范围内与各自峰面积积分值呈良好线性关系(r均为0.9999);平均加样回收率分别为99.36%和99.06%,RSD分别为3.21%和3.96%(n均为6)。结论:本方法简便、准确、重复性好,可用于提高青蒿的质量控制方法。
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| 关 键 词： | 青蒿 樟脑 冰片 毛细管气相色谱法 含量测定 |
Content Determination of Camphor and Borneol in Artemisia annua from Different Areas by Capillary GC |
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| WANG Huai-yuMianyang Normal College,Sichuan Mianyang,China PENG Rui,MA Peng.Content Determination of Camphor and Borneol in Artemisia annua from Different Areas by Capillary GC[J].China Pharmacy,2012(15):1395-1396. |
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| Authors: | WANG Huai-yuMianyang Normal College Sichuan Mianyang China PENG Rui MA Peng |
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| Affiliation: | (Chongqing Academy of Chinese Materia Media,Chongqing 400065,China) WANG Zheng-fan(Weinan Normal College,Shaanxi Weinan 714000,China) |
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| Abstract: | OBJECTIVE:To establish the method for the content determination of camphor and borneol in Artemisia annua.METHODS:Capillary GC method was adopted.The determination was carried out on a HP-5 capillary column(30 m× 0.25 mm×0.25 μm) at the flow rate of 1.2 mL·min-1.FID detector was used and the temperature of detector was 280 ℃.The injector temperature was 250 ℃ with temperature programming.The split ratio was 20:1.RESULTS:The linear ranger were 1.16～46.4 μg·mL-1 for camphor and 1.24～49.6 μg·mL-1(r=0.999 9)for borneol respectively.The average recoveries were 99.36%(RSD=3.21%,n=6) and 99.06%(RSD=3.96%,n=6).CONCLUSION:The method is simple,accurate and reliable,and it can be used for quality control of A.annua. |
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| Keywords: | Artemisia annua Camphor Borneol Capillary GC Content determination |
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