炮制对黄柏中3种生物碱含量的影响

目的:考察炮制对黄柏中3种生物碱含量的影响。方法:选用高效液相色谱(HPLC)法测定3种炮制(酒炙、盐炙和炒炭)品中3种黄柏生物碱的含量。色谱柱为迪马C18(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸(48∶52,每100mL含十二烷基磺酸钠0.1g),检测波长为265nm,流速为1.0mL·min-1,柱温为室温,进样量为10μL。结果:盐酸小檗碱、盐酸巴马汀和盐酸药根碱含量在炮制后普遍降低,以炒炭最为显著;也有个别特例。结论:黄柏在炮制后(酒炙品、盐炙品)仍然可以选择3种生物碱作为质量控制的指标。本方法简单、快速、准确,适用于同时测定黄柏炮制品中3种生物碱的含量。

Effect of Processing Method on the Content of 3 Kinds of Alkaloids in Phellodendron chinense

OBJECTIVE：To study the influence of processing method on the content of 3 kinds of alkaloids in Phellodendron chinense. METHODS：HPLC was used to determine the content of 3 kinds of alkaloids in P. chinense,such as stir-frying with wine,salt-roast process and carbonization. The separation was performed on Zorbax SB-C18（250 mm×4.6 mm,5 μm） column. The mobile phase consisted of acetonitrile-0.1% phosphoric acid （48 ∶ 52,0.1 g SDS per 100 mL） with flow rate of 1.0 mL·min-1. Detection wavelength was set at 265 nm and column temperature was set at room temperature. Injection volume was 10 μL. RESULTS：Except for special case,the content of berberine hydrochloride,pamatine hydrochoride and jateorizine hydrochoride were all decreased after processing,especially carbonization.CONCLUSION：The 3 kinds of alkaloids can be also used as index of quality control of processed P. chinense. The method is simple,rapid and accurate for the determination of 3 kinds of alkaloids in P. chinense.