清咽滴丸6种成分提取物体外抗柯萨奇病毒B3、B5的作用研究

目的:研究清咽滴丸6种中药成分提取物体外抗柯萨奇病毒B3、B5(CVB3、CVB5)的作用。方法:以Vero细胞为模型,通过观察药物毒性和病毒致细胞病变效应,用MTT法辅助检测细胞活性情况,检验药物的毒性和药物的抗病毒效应。结果:清咽滴丸的6种主要成分中只有甘草和诃子既可以抑制病毒侵入Vero细胞,又能治疗被CVB3、CVB5感染的Vero细胞。结论:甘草、诃子提取物可保护Vero细胞,抑制病毒在细胞内增殖,并具有一定的抗CVB3、CVB5感染的能力。

Study on Antiviral Effect of Aqueous Extracts from the 6 Components of Qingyan Dripping Pills on Coxsackie Virus Type B3 and B5 in Vitro

OBJECTIVE：To investigate the effects of aqueous extracts from 6 components of Qingyan dripping pills on coxsackie virus type B3（CVB3）and B5（CVB5）in vitro.METHODS：Using vero cell as a model,the antiviral effects of the aqueous extracts from 6 components of Qingyan dripping pills on CVB3,CVB5 and their cytotoxicity on Vero cells were studied by CPE and MTT method.Antiviral mechanism of the aqueous extracts was also investigated by two different ways.RESULTS：Baked licorice and Terminalia chebula can protect Vero cells from CVB3 and CVB5 by inhibiting the virus of entering into cells and curing the sick cells.CONCLUSION：The aqueous extracts from B.licorice and T.chebula inhibit the proliferation of CVB3 and CVB5 to protect Vero cells.They also have antiviral effect against CVB3 and CVB5.