| RP-HPLC法测定山豆根中三叶豆紫檀苷的含量 |
| |
| 作者姓名： | 邓放 吴兵 董小萍 |
| |
| 作者单位： | 成都中医药大学药学院 中药材标准化教育部重点实验室 中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都,610075 |
| |
| 基金项目： | 成都中医药大学校基金课题(课题编号:ZRYB200415) |
| |
| 摘 要： | 目的:建立RP-HPLC测定山豆根中三叶豆紫檀苷含量的方法.方法:色谱柱为Symmetry shield RP C18柱,流动相为甲醇-水(50:50),检测波长为310 nm.结果:三叶豆紫檀苷在0.49 ～ 4.9μg范围内与峰面积有良好的线性关系；精密度试验相对标准偏差为1.31％；加样回收率为96.69％～98.57％;山豆根中三叶豆紫檀苷含量为0.019％.结论:本法操作简便,结果准确,可用于山豆根中三叶豆紫檀苷含量的测定.
|
| 关 键 词： | 反相高教液相色谱 山豆根 三叶豆紫檀苷 |
Determination of trifolirhizin in Radix et Rhizoma sophorae tonkinensis by RP-HPLC |
| |
| Authors: | DENG Fang WU Bing DONG Xiao-ping |
| |
| Affiliation: | (Pharmacy College, Chengdu University of Traditional Chinese Medicine; The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine; State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu 610075, China) |
| |
| Abstract: | Objective: To develop a method to determine trifolirhizin in Radix et Rhizoma sophorae tonkinensis by RP-HPLC. Method: The analytical column was symmetry shield RP C18 column.The mobile phase was methanol-water(50:50). Result: The detection wavelength was set at 310 nm. The calibration curve was linear in the range of 0.49 μg～4.9μg for trifolirhizin. The relative standard deviation (RSD) of precision test was 1.31%.The recovery was between 96.69% and 98.57%. Trifolirhizin contents in Radix et Rhizoma sophorae tonkinensis was 0.019%. Conclusion: The method is simple, quick, accurate,and reliable for the determination of trifolirhizin in Radix et Rhizoma sophorae tonkinensis. |
| |
| Keywords: | RP-HPLC Radix et Rhizoma Sophorae tonkinensis trifolirhizin |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
