HPLC法测定五维葡钙口服溶液中四组份的含量

目的:建立HPLC法同时测定五维葡钙口服溶液中维生素B1,维生素B2,维生素B6(以下简称VB1,VB2,VB6)和烟酰胺的含量.方法:采用C18柱；以0.005 mol·L-1己烷磺酸钠与0.008 mol·L-1庚烷磺酸钠的1％乙酸溶液-甲醇(74:26)为流动相；检测波长276 nm；流速:1.0 mL· min-1.结果:VB1在36.30～84.70 μg·mL-1；VB2在7.26～ 16.94 μg·mL-1；VB6在10.93～25.51μg·mL-1与烟酰胺在36.20～84.48 μg·mL -1的范围内峰面积与浓度具有良好线性关系；VB1,VB2,VB6与烟酰胺的回收率依次为:99.78％,RSD=1.58％(n=6)；99.80％,RSD=1.22％ (n =6)；100.07％,RSD=0.75％ (n=6)；99.62％,RSD=1.01％ (n =6).结论:该方法准确,灵敏度高,重现性好.

Determination of the Content of Four components in Five Vitamins and Calcium Gluconate Oral Solution by HPLC

Objective：To establish an HPLC method for the determination of Vitamin Bj, Vitamin B2, Vitamin B6 and Nicotinamide in Five Vitamins and Calcium Gluconate Oral Solution. Methods： Column：Shimadzu Cis; Mobile phase： 1% acetic acid solution of 0. 005 mol ·L^-1 Hexanesulfonic acid Sodium and 0. 008 mol · L^-1 Heptanesulfonic acid Sodium-methanol （74： 26） ; Detective wavelength： 276nm; Flow rate： 1.0 mL · min^-1. Results：The calibration curve was linear in the range of 36. 30 -84. 70μg ·mL^-1, 7.26 - 16. 94 μg · mL-1, 10. 93 -25. 51 μg·mL^-1, 36. 20 -84. 48 μg·mL^-1 for Vitamin Bj, Vitamin B2, Vitamin B6 and Nicotinamide; The recovery rate of Vitamin B~ , Vitamin B2, Vitamin B6 and Nieotinamide was 99.78%, RSD 1.58% （n =6） ; 99. 80%, RSD 1.22% （n =6） ; with 100. 07%, RSD O. 75% （n =6） ; 99.62%, RSD 1.01% （n =6）. Conclusion：The method is simple, quick, accurate and reliable.