虎杖苷对大鼠皮肤创伤愈合的治疗作用的实验研究

目的 探讨虎杖苷对大鼠皮肤急性创面愈合的治疗作用及可能机制.方法 54只大鼠分为三组:正常组(n=6)、对照组(n=24)及虎杖苷组(n=24).正常组为无处理,建立背部急性全层皮肤缺损模型后虎杖苷组予以虎杖苷20 mg/kg治疗10天,对照组大鼠予以等量生理盐水治疗.统计各组大鼠创面愈合时间、愈合率,HE染色观察创面形态学变化,试剂盒检测创面组织Ⅰ型、Ⅲ型胶原蛋白,TUNEL法检测创面组织细胞凋亡,westem blot法检测创面组织Bax及Bcl-2表达,荧光法检测创面组织caspase-3活性.结果 与对照组比较,虎杖苷组创面愈合率明显提高,愈合时间明显缩短;与对照组比较,虎杖苷组创伤组织中Ⅰ型胶原含量比同期对照组低,而Ⅲ型胶原含量比同期对照组高;与对照组比较,虎杖苷组凋亡细胞数减少,Bax表达降低,Bcl-2表达增加,caspase-3活性减低.结论 虎杖苷治疗可以促进创面愈合,其机制可能与抑制细胞凋亡有关.

Experimental study on the effect of polydatin on skin wound healing of the rats

Objective To investigate the effect of polydatin in treating skin injury of the rats.Methods 54 rats were randomly divided into normal group (n=6),control group (n=24),and polydatin group (n=24).To induce skin injury,full-thickness excisional lesion was performed on the rats' back.No injury was inflicted in normal group.The rats in polydatin group received 20 mg/kg of polydatin for 10 days,while the rats in control group received the same amount of normal saline.The healing ratio and healing time were calculated;morphological changes were observed by HE staining;type Ⅰ and Ⅲ collagen protein were detected by a commercial kit;apoptosis was detected by TUNEL kit;Bax and Bcl-2 expression were detected by western blot;caspase-3 activity was detected by fluorescence method.Results Compared with control group,the obviously increased healing ratio and decreased healing time were observed in polydafin group;compared with control group,the decreased type I collagen protein and the increased type m collagen protein were detected in polydatin group;compared with control group,the decreased apoptotic cells,Bax down-regulation,Bcl-2 up-regulation,and the decreased caspase-3 activity were detected in polydatin group.Conclusions Polydatin promotes the skin wound healing in rats.This protective effect of polydatin is at least partially mediated through the inhibition of apoptosis.