血清AMA-M2亚型、调节性T细胞与IL在PBC患者中的表达及临床意义

目的 研究原发性胆汁性肝硬化（PBC）患者血清抗线粒体抗体亚型M2（autimitochondria antibody typeⅡ,AMA-M2）、调节性T细胞和相关白介素（IL）的表达及相关性,以探讨其诊断价值.方法 分别收集PBC确诊患者组（42例）、疾病对照组（36例）和健康对照组（50例）的血清样本,采用免疫印迹法检测AMA-M2;流式细胞术检测CD4±CD25＋调节性T细胞和CD4＋、CD8＋、CD4＋、CD8＋水平;IL-2、IL-4、IL-6、IL-8、IL-10采用ELISA定量方法测定.结果 PBC组、健康对照组和疾病对照组AMA-M2阳性率差异有统计学意义（P＜0.05）.与AMA-M2阴性组比较,阳性组CD4＋、CD4＋/CD8＋分别增加11.57％和72.79％,CD4＋CD25＋、CD8＋水平分别降低61.50％和35.97％,IL-2、IL-4、IL-6、IL-8、IL-10分别升高189.65％、24.29％、2184.54％、230.33％和1281.37％,且差异均有统计学意义（P＜0.05）;但与弱阳性组相比,阳性组血清中只有CD4＋、CD8＋、IL-4、IL-6、IL-8、IL-10差异有统计学意义（P＜0.05）.结论 PBC患者免疫功能异常与IL-2、IL-6、IL-8、IL-10高水平表达相关,血清AMA-M2阳性检测、外周血调节性T细胞比例及细胞因子浓度的检测可为临床诊断PBC提供依据.

Expressions of anti-mitochondrial M2 antibodies,regulatory T cells,and relevant interleukin in peripheral blood of patients with primary biliary cirrhosis and its clinical significance

Objective To study the expressions and correlation of serum anti-mitochondrial M2 antibodies （auti-mitochondria antibody type Ⅱ,AMA-M2）,regulatory T cells,and relevant interleukin （IL） in patients with primary biliary cirrhosis （PBC）,and to explore the diagnostic value.Methods The serum samples of the PBC diagnosed patients group （n=42）,disease control group （n=36）,and healthy controls （n=50） were collected respectively.Western Blot Method was used to detect AMA-M2 and the CD4＋CD25＋ regulatory T cells and the levels of CD4＋,CD8＋,CD4＋/CD8＋ were examined by the Flow Cytometry.The ELISA quantitative method was used to determinate IL-4,IL-6,IL-2,IL-8,and IL-10.Results There were statistical differences in the positive rate of AMA-M2 hetween the PBC group,the healthy controls,and the disease control group （P 〈 0.05）.Compared with the AMA-M2 negative group,the levels of CD4＋ and CD4＋/CD8＋ were increased by 11.57% and 72.79% respectively,the CD4＋CD25＋ and CD8＋ levels were decreased by 61.50% and 35.97% respectively,IL-2,IL-4,IL-6,IL-8,and IL-10 were increased by 189.65%,24.29%,2184.54%,230.33%,and 1281.37% respectively in the positive group,with statistical differences （P 〈 0.05）.But in comparison with the weak positive group,there were statistical differences in only CD4＋/CD8＋,IL-4,IL-6,IL-8,and IL-10 between the weak positive and positive groups （P 〈 0.05）.Conclusions The immune dysfunction of the patients with PBC correlates with high expression levels of IL-2,IL-6,IL-8,IL-10.Positive serum AMA-M2 detection,the proportion of regulatory T cell in peripheral blood,and the detection of cytokine concentration can provide references for clinicaly diagnosing PBC.