| HPLC法测定盐炙前后知母中新芒果苷、芒果苷和异芒果苷 |
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| 引用本文： | 宋泽璧,吴莹,高慧.HPLC法测定盐炙前后知母中新芒果苷、芒果苷和异芒果苷[J].现代药物与临床,2015,30(2):145-148. |
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| 作者姓名： | 宋泽璧 吴莹 高慧 |
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| 作者单位： | 辽宁中医药大学 药学院，辽宁 大连 116600; 国家中医药管理局中药炮制原理解析重点研究室，辽宁 大连 116600; 辽宁省中药炮制工程技术研究中心，辽宁 大连 116600 |
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| 基金项目： | 国家自然科学基金资助项目(81102810)；教育部高等学校博士学科点专项科研基金资助项目 |
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| 摘 要： | 目的建立HPLC法同时测定盐炙前后知母中新芒果苷、芒果苷、异芒果苷,并比较盐炙前后3种成分的差异。方法采用HPLC法,Ecosil色谱柱(150 mm×4.6 mm,5μm),流动相乙腈–0.2%冰醋酸,梯度洗脱,体积流量1.0 m L/min,检测波长258 nm,柱温室温,进样量10μL。结果新芒果苷、芒果苷、异芒果苷分别在0.860 0~17.200 0μg、0.806 5~16.130 0μg、0.039 0~0.780 0μg与峰面积的线性关系良好;平均回收率分别为98.32%、98.30%、97.27%,RSD值分别为0.87%、0.74%、0.71%(n=6)。知母盐炙后新芒果苷、异芒果苷的量减少,而芒果苷的量增加。结论该方法准确、专属性强、灵敏度高,可用于知母中新芒果苷、芒果苷、异芒果苷的质量控制方法。新芒果苷、芒果苷之间可能在炮制过程中发生了转化,可为进一步研究盐知母炮制机制提供参考。
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| 关 键 词： | 知母 盐炙 芒果苷 新芒果苷 异芒果苷 高效液相色谱 |
| 收稿时间： | 2014/5/19 0:00:00 |
Determination of neomangiferin, mangiferin, and isomangiferin in Anemarrhenae Rhizoma before and after stir-frying with salt-water by HPLC |
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| SONG Ze-bi,WU Ying and GAO Hui.Determination of neomangiferin, mangiferin, and isomangiferin in Anemarrhenae Rhizoma before and after stir-frying with salt-water by HPLC[J].Drugs & Clinic,2015,30(2):145-148. |
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| Authors: | SONG Ze-bi WU Ying and GAO Hui |
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| Affiliation: | College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China
;Key Laboratory of Processing Theory Analysis of State Administration of Traditional Chinese Medicine, Dalian 116600, China;Engineering Technology Research Center of Traditional Chinese Medicine processing of Liaoning Province, Dalian 116600, China;College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China
;Key Laboratory of Processing Theory Analysis of State Administration of Traditional Chinese Medicine, Dalian 116600, China;Engineering Technology Research Center of Traditional Chinese Medicine processing of Liaoning Province, Dalian 116600, China;College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China
;Key Laboratory of Processing Theory Analysis of State Administration of Traditional Chinese Medicine, Dalian 116600, China;Engineering Technology Research Center of Traditional Chinese Medicine processing of Liaoning Province, Dalian 116600, China |
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| Abstract: | |
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| Keywords: | Anemarrhenae Rhizoma stir-frying with salt-water mangiferin neomangiferin isomangiferin HPLC |
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