HPLC同时测定穿心莲药材及其注射液中3种内酯类成分的含量

目的利用HPLC同时测定穿心莲药材及其注射液中穿心莲内酯、脱水穿心莲内酯和新穿心莲内酯的含量。方法色谱柱为Shimadzu Shim-pack VP-ODS柱(4.6 mm×150 mm,5μm),以甲醇-水(53∶47)洗脱,流速:1 mL.min?1,柱温:25℃,检测波长225 nm处检测穿心莲内酯,检测波长205 nm处检测脱水穿心莲内酯与新穿心莲内酯。结果穿心莲药材及其注射液中3种内酯类成分能达到很好分离,线性关系良好(r≥0.999 5);平均加样回收率为99.6%~101.2%,RSD<3%。结论本方法灵敏、准确、可靠、重复性好,可用于穿心莲药材及其注射液的质量评价与3种内酯类成分的快速测定。

Simultaneous Determination of 3 Components of Diterpenoids in Andrographis Paniculata and Chuanxinlian Injection by HPLC

OBJECTIVE To develop a quantitative method for simultaneous determination of andrographolide,deoxyandrographolide and neoandrographolide in Andrographis paniculata and Chuanxinlian injection by HPLC.METHODS A Shimadzu Shim-pack VP-ODS C18 column(4.6 mm×150 mm,5 μm) was used with CH3OH and water(53∶47) as mobile phase.The flow rate was 1 mL·min 1,the column temperature was 25 ℃,the detection wavelength of andrographolide was set at 225 nm and the detection wavelength of deoxyandrographolide and neoandrographolide was set at 205 nm.RESULTS The 3 active components of diterpenoids were in baseline separation.The linearity of this method was good(r≥0.999 5),and the average recoveries were 99.6% 101.2%,RSD<3%.CONCLUSION The method is sensitive,accurate,reliable and repeatable,which can be used for quality evaluation of Andrographis paniculata and its injection,and for determination of 3 active components of diterpenoids quickly.