五磨饮对脾虚气滞证功能性消化不良大鼠脑肠肽的影响

目的研究五磨饮对脾虚气滞证功能性消化不良(functional dyspepsia,FD)大鼠脑肠肽的影响,探讨其作用机制。方法除正常组外,其余大鼠采用“慢性束缚+游泳+饮食不节”复合因素法制备脾虚气滞证FD模型。按体质量随机分为模型组、多潘立酮组、柴芍六君子汤组、五磨饮高剂量组(6.2 g·kg^-1)、中剂量组(3.1 g·kg^-1)、低剂量组(1.55 g·kg^-1)。分组后,除正常组及模型组外,其余大鼠灌胃给药干预14 d。ELISA法检测血浆胃促生长素(Ghrelin)、胃动素(motilin,MTL)、胃泌素(gastrin,GAS)、胆囊收缩素(cholecystokinin,CCK)、血管活性肠肽(vasoactive intestinal peptide,VIP)、降钙素基因相关肽(calcitonin gene related peptide,CGRP)含量,免疫组化法检测胃窦及下丘脑组织Ghrelin、MTL、GAS、CCK、VIP、CGRP表达。结果与模型组比较,五磨饮各剂量组大鼠血浆Ghrelin、MTL含量显著增加(P<0.01或P<0.05),CCK含量显著降低(P<0.01),高剂量组和中剂量组大鼠血浆VIP含量明显降低(P<0.05),高剂量组大鼠血浆CGRP含量明显降低(P<0.05);各剂量组大鼠胃窦组织Ghrelin、MTL、GAS、CCK、VIP、CGRP的表达差异没有统计学意义;高剂量组大鼠下丘脑组织Ghrelin表达显著增加(P<0.01),中剂量组大鼠下丘脑组织Ghrelin、MTL、GAS、VIP、CGRP表达显著增加(P<0.01)。结论五磨饮具有调节脾虚气滞证FD大鼠脑肠肽表达的作用,这可能是其作用机制之一。

Effect of Wumoyin Decoction on Regulating Brain-gut Peptide in Functional Dyspepsia Rats with Spleen Deficiency and Qi Stagnation Syndrome

OBJECTIVE To study the effect of Wumoyin decoction on regulating functional dyspepsia rats with spleen deficiency and qi stagnation syndrome, explore its mechanism of action. METHODS Except the normal group, the other rats were modeled by "chronic restraint+swimming+irregular eating" compound factor method. The rats were randomly divided into model group, domperidone group, Chaishao Liujunzi decoction group, Wumoyin decoction high dose group(6.2 g·kg^-1), middle dose group(3.1 g·kg^-1) and low dose group(1.55 g·kg^-1). Besides the normal group and model group, the other groups of rats were given the corresponding drug intervention for 14 d. The content of Ghrelin, MTL, GAS, CCK, VIP, CGRP in plasma were detected by ELISA method, and the expression of Ghrelin, MTL, GAS, CCK, VIP, CGRP in gastric antrum tissue and hypothalamus were detected by immunohistochemistry method. RESULTS Compared with the model group, the Ghrelin and MTL in plasma of rats in Wumoyin decoction each dose group was significantly raised(P<0.01), and the CCK was very significantly reduced(P<0.01). The VIP in plasma of rats in the high dose and middle dose groups were significantly reduced(P<0.05). The CGRP in plasma of rats in the high dose group was significantly reduced(P<0.05). There was no significant difference in the expression of Ghrelin, MTL, GAS, CCK, VIP and CGRP in gastric antrum of rats in each dose group. The expression of Ghrelin in the hypothalamus of the rats in the high dose group was significantly increased(P<0.01). The expressions of Ghrelin, MTL, GAS, VIP, CGRP in the hypothalamus of rats in the middle dose group were significantly increased(P<0.01). CONCLUSION Wumoyin decoction has the effect of regulating the expression of brain gut peptide in rats with functional dyspepsia of spleen deficiency and qi stagnation syndrome, which may be one of its mechanisms.