HPLC法同时测定脊痛宁胶囊中葛根素和芍药苷的含量

目的:建立脊痛宁胶囊中葛根素和芍药苷的含量测定方法。方法:采用高效液相色谱法,色谱柱为Diamonsil C18(2)柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.5%磷酸溶液(13.5∶86.5),流速为1.0 mL.min-1,检测波长为230 nm,柱温为35℃。结果:葛根素在88.512～885.120μg.mL-1范围内线性关系良好(r=0.999 8),平均加样回收率为99.90%,RSD=1.28%(n=6);芍药苷在49.28～492.80μg.mL-1范围内线性关系良好(r=0.999 9),平均加样回收率为99.42%,RSD=1.02%(n=6)。结论:本方法简便、准确,专属性强,测定结果重复性好,是脊痛宁胶囊中葛根素和芍药苷同时定量分析的有效方法。

Simultaneous determination of puerarin and paeoniflorin in Jitongning capsule by HPLC

Objective： To establish the HPLC method for the simultaneous determination ofpuerarin and paeonifiorin in Jitongning capsule. Methods： HPLC was carried out on a Diamonsil C18column （250 mm × 4.6 mm, 5 μm）, the mobile phase consisted of acetonitrile-0.5% phosphoric acid solution （13.5 ： 86.5） at the flow rate of 1.0 mL.min~, the detection wavelength was 230 mn, and the column temperature was 35 ℃. Results： The linear range of puerarin was 88.512 - 885.120 μg·mL^-1（r = 0.999 8）. The average recovery was 99.90% with RSD of 1.28% （n = 6）. The linear range of paeoniflorin was 49.28 - 492.80 μg·mL^-1 （r = 0.999 9）, the average recovery was 99.42% with RSD of 1.02% （n = 6）. Conclusion： This method is simple and accurate, with good specificity and repeatability, and can be used for quantitative control of Jitongning capsule.