米格列醇关键中间体6-(N-羟乙基)-6-脱氧-氨基山梨糖的生物合成工艺优化

目的:优化米格列醇关键中间体6-(N-羟乙基)-6-脱氧-氨基山梨糖的生物合成工艺。方法:以N-羟乙基葡糖胺作为原料,采用单因素试验方法优化工艺。分别考察了温度、转化时间、静息细胞浓度、底物浓度及起始pH对氧化葡萄糖酸杆菌静息细胞生物催化合成6-脱氧-6-羟乙基氨基山梨糖的影响。结果:在静息细胞浓度10%、底物浓度15%、起始pH 7.0,1000 mL摇瓶装液150 mL,30℃,200 r.min-1条件下振荡转化10 h,底物转化率可达93%。结论:该生物转化工艺切实可行。

Biosynthesis of key intermediate 6-(2-hydroxyethyl) amino-6-deoxy-L-sorbose of miglitol

Objective: To optimize the biosynthesis process of the key intermediate 6-(2-hydroxyethyl) amino-6-deoxy-L-sorbose of miglitol.Methods: Using N-(2-hydroxyethyl)-glucamine as starting material,single factor experiment design was applied to optimize the process.The effects of temperature,time,the concentration of resting cells,the concentration of substrate and the starting pH on biosynthesis of 6-(2-hydroxyethyl) amino-6-deoxy-L-sorbose by Gluconobater oxydans’s resting cells were studied.Results: The transformation ratio could reach 93% when the concentration of resting cells and N-(2-hydroxyethyl)-glucamine were 10% and 15% respectively,the initial pH was 7.0 with 150 mL of medium being put into a flask of 1 000 mL,the temperature was 30 ℃,and cultivation time was 10 h shaken at 200 r·min-1.Conclusion: This biotransformation process is practicable.