| 10种黄酮类化合物对糖尿病致病机制中重要通路的抑制作用 |
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| 引用本文： | 程丽艳,史红.10种黄酮类化合物对糖尿病致病机制中重要通路的抑制作用[J].中国新药杂志,2010(9). |
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| 作者姓名： | 程丽艳 史红 |
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| 作者单位： | 浙江省医学科学院; |
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| 基金项目： | 浙江省中医药管理局青年基金(2007YA022) |
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| 摘 要： | 目的:观察10种黄酮类化合物对糖尿病致病机制中4条重要通路的多靶点抑制作用,发挥中药多靶点、综合治疗的优势。方法:从正常大鼠脑组织中提取半纯化的蛋白激酶C(PKC)、肾脏组织中提取醛糖还原酶(AR)粗酶,Fenton试剂体外激发正常大鼠脑组织脂质过氧化生成丙二醛(MDA)及体外合成高级糖化终产物(AGEs),将上述酶或合成物与10种黄酮类化合物(终浓度为1 mg.mL-1)作用,观察黄酮类化合物对上述酶或合成物的抑制作用。结果:黄芩苷和灯盏花素对AR,PKC,AGEs及MDA均有不同程度的抑制作用。结论:上述结果提示此两种黄酮类化合物可以通过抑制上述4条通路来预防糖尿病及其并发症的发生,为进一步的机制研究及临床应用提供良好的实验依据。
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| 关 键 词： | 活性黄酮 糖尿病 并发症 |
Inhibitory effects of 10 flavonoid glycosides on the pathways of diabetic pathogenesis |
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| CHENG Li-yan,SHI Hong.Inhibitory effects of 10 flavonoid glycosides on the pathways of diabetic pathogenesis[J].Chinese Journal of New Drugs,2010(9). |
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| Authors: | CHENG Li-yan SHI Hong |
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| Affiliation: | CHENG Li-yan,SHI Hong(Zhejiang Academy of Medical Sciences,Hangzhou 310013,China) |
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| Abstract: | Objective:To estimate the inhibitory effects of 10 flavonoid glycosides on the 4 important pathways in diabetic pathogenesis,and to find new and effective extracts of Chinese herbs for treatment of diabetes.Methods:Semi-purified protein kinase C(PKC) was extracted from brains of normal rats,and crude aldose reductase(AR) was from kidneys.Malondialdehyde(MDA) was produced by activating brain tissue with Fenton reagent.Advanced glycation end products(AGEs) were synthetized in vitro.The effects of flavonoid gl... |
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| Keywords: | active flavanoid diabetes complication |
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