| UPLC法测定痤疮凝胶中黄芩苷和蒙花苷的含量 |
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| 引用本文： | 范丽丽,李楠,顾星,朱蕴,刘志东,夏庆梅,郭丽丽,王爱潮.UPLC法测定痤疮凝胶中黄芩苷和蒙花苷的含量[J].天津中医药大学学报,2015,34(1):42-44. |
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| 作者姓名： | 范丽丽 李楠 顾星 朱蕴 刘志东 夏庆梅 郭丽丽 王爱潮 |
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| 作者单位： | 天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 300193;天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 300193;天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 300193;天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 300193;天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 300193;天津中医药大学, 天津 300193;天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 300193;天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 300193 |
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| 基金项目： | 教育部新世纪优秀人才支持计划(NCET-12-1068). |
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| 摘 要： | 目的]建立痤疮凝胶中黄芩苷、蒙花苷含量测定方法。方法]采用Acquity BEH C18(2.1 mm×50 mm,1.7μm)色谱柱;以乙腈-0.1%甲酸(20∶80)为流动相,流速0.3 m L/min,检测波长320 nm,柱温35℃。结果]黄芩苷和蒙花苷分别在3.767~75.34μg和0.840 96~33.638 4μg范围内线性关系良好,平均回收率分别为99.04%和97.42%(n=6);RSD分别为1.97%和1.81%。结论]该方法简便、准确、重现性良好,为痤疮凝胶的质量控制提供了理论依据。
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| 关 键 词： | 痤疮凝胶 黄芩苷 蒙花苷 超高效液相色谱法 含量测定 |
| 收稿时间： | 2014/11/7 0:00:00 |
Determination of Baicalin and Linarin in Cuochuang Gel by UPLC |
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| FAN Li-li,LI Nan,GU Xing,ZHU Yun,LIU Zhi-dong,XIA Qing-mei,GUO Li-li and WANG Ai-Chao.Determination of Baicalin and Linarin in Cuochuang Gel by UPLC[J].Journal of Tianjin University of Traditonal Chinese Medicine,2015,34(1):42-44. |
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| Authors: | FAN Li-li LI Nan GU Xing ZHU Yun LIU Zhi-dong XIA Qing-mei GUO Li-li and WANG Ai-Chao |
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| Affiliation: | Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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| Abstract: | |
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| Keywords: | Cuochuang Gel baicalin Linarin UPLC content determination |
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