基于网络药理学探讨女贞子治疗动脉粥样硬化的作用机制

目的] 基于网络药理学和生物信息学方法分析女贞子治疗动脉粥样硬化的分子生物学机制。方法] 基于TCMSP数据库与文献挖掘女贞子中化学成分; 利用PharmMapper网站预测女贞子化学成分靶点, 构建"成分-靶点"网络; 利用TTD、DisGeNET、DrugBank数据库筛选与AS疾病作用相关靶点。利用Cytoscape软件对化合物靶点和AS疾病靶点进行网络拓扑分析。利用STRING数据库对共有靶点构建PPI网络, 进行GO分析和KEGG分析。采用油红O染色和检测细胞内总胆固醇含量评价红景天苷和毛蕊花糖苷抗动脉粥样硬化活性。结果] "成分-AS疾病靶点"网络显示14个活性成分和170个疾病靶点; 女贞子化学成分和动脉粥样硬化共同靶点9个; GO分析确定352个条目, KEGG通路富集结果确定了14条与AS相关的通路。细胞实验显示红景天苷和毛蕊花糖苷显著减少泡沫细胞的形成, 降低细胞内胆固醇含量。结论] 女贞子中苯乙醇苷类化合物可能通过调节AMPK、PPAR信号通路调节胆固醇代谢发挥抗动脉粥样硬化作用。

Study on anti-atherosclerotic effect of Ligustrum lucidum based on network pharmacology

Objective] To analyze the molecular biological mechanism of Ligustrum lucidum in the treatment of atherosclerosis based on network pharmacology and bioinformatics methods.Methods] Acquire the Ligustrum lucidum active chemical ingredients based on traditional Chinese medicines systems pharmacology and literature. The targets of LLF were screened by uploading the chemical structure to PharmMapper database, establish "constituent-target" network. Screening atherosclerosis related targets through TTD, DrugBank and DisGeNET databases. The mutual target proteins was analyzed by STRING database to perform GO biological process analysis and KEGG pathway enrichment analysis. The antiatherosclerotic activity of salidroside and cilioside was evaluated by oil red O staining and intracellular total cholesterol detection.Results] The "Constituent-Target" network display a total of 14 active chemical constituent and 170 potential disease targets were obtained, and there were 9 common targets of Ligustrum lucidum chemical components and atherosclerosis. GO biological process analysis identified 352 terms and the result of KEGG enrichment analysis showed 14 pathways were associated with atherosclerosis. Cell experiments showed that salidroside and acteoside significantly reduced the formation of foam cells and the content of intracellular total cholesterol.Conclusion] The phenylethanol glycosides may play an anti-atherosclerosis role by regulating AMPK and PPAR signaling pathways to regulate cholesterol metabolism.