nm23-H1基因遗传不稳定性和宫颈癌的相关性研究

目的 分析宫颈癌及癌前病变中nm23-H1基因遗传不稳定性及其与宫颈癌临床病理特征的相关性,探讨nm23-H1基因在宫颈癌发生、发展中的作用.方法 收集手术及活检宫颈新鲜组织标本146例,包括宫颈上皮内瘤变（cervical intraepithelial neoplasia,CIN）Ⅱ～Ⅲ 级48例,宫颈鳞癌（squamous cell carcinoma,SCC）48例,慢性宫颈炎50 例.采用免疫组化技术检测nm23-H1蛋白,同时用PCR-SSCP法检测nm23-H1基因遗传不稳定性.结果 （1） nm23-H1蛋白表达率随着宫颈肿瘤的进展逐渐减弱.（2） nm23-H1 基因D17S396位点的杂合性缺失（loss of heterozysity,LOH）发生率在宫颈癌晚期较多见,而微卫星不稳定（microsatellite instability,MSI）在癌前病变及早期癌中较多见.（3） LOH的增加与nm23-H1蛋白的低表达有关,MSI对nm23-H1蛋白表达的影响不大.结论 nm23-H1基因可能参与了宫颈癌的进展及浸润转移,nm23-H1基因的MSI和 LOH可能通过不同的机制影响肿瘤进展.

Genetic instability of nm23-H1 gene in cervical cancer

Objective To investigate nm23-H1 gene instability in cervical cancer and its correlation with clinical and pathological characteristics. Methods A total 146 cervical tissue samples were collected from operation and biopsy, including 48 cases of CIN Ⅱ～Ⅲ, 48 cases of SCC, 50 cases of chronic cervicitis. The nm23-H1 proteins were detected by immunohistochemical method. The nm23-H1 gene instability （include LOH and MSI） were examined by PCR-SSCP. The correlation of nm23-H1 gene instability with clinical and pathological characteristics of cervical cancer were anylyzed. Results The expression of nm23-H1 protein decreased with the progressing of cervical tumor. The LOH in nm23-H1 gene was more frequent in later stage cervical cancer. MSl was more frequent in CIN and early stage cervical cancer. The increase of LOH was related to the decreased nm23-H1 protein expression. Conclusion MSI and the LOH of nm23-H1 gene are detected in cervical cancer, which may be involved in the progress in cervical cancer with different mechanicals.