ER-β、MEK-2、p-ERK在子宫内膜异位症组织中的表达及意义

目的:探讨雌激素受体ER-β与细胞信号传导通路丝裂原活化蛋白激酶(MAPK)上游激活酶MEK-2和下游激活酶pERK在子宫内膜异位症(EMs)发病机制中的作用.方法:采用免疫组化S-P法检测46例EMs患者在位及异位内膜、40例正常内膜组织中ER-13、MEK-2和p-ERK的蛋白表达.结果:ER-β、MEK-2和p-ERK在异位内膜和在位内膜中表达高于正常内膜,差异有显著性意义(P＜0.01),异位内膜表达高于在位内膜,差异具有显著意义(P＜0.01)；ER-β和MEK-2、p-ERK在正常子宫内膜、在位内膜和异位内膜中表达呈正相关( r=0.628,P＜0.01；r=0.559,P＜0.01).MEK-2和p-ERK在正常子宫内膜、在位内膜和异位内膜中表达呈正相关(r=0.699,P＜0.01).结论:ER-β、MEK-2和p-ERK在EMs组织中高表达并呈正相关,MAPK信号通路可能与ER-β相互作用,在EMs的发生发展中起着重要作用.

Expression of ER-β, MEK-2 and p-ERK in endometriosis

Objective: To detect the expression of estrogen receptor ER-beta and MAPK upstream activate enzymes MEK-2 and downstream activate enzymes p-ERK in the pathogenesis of endometriosis(EM).Methods: Immunohistochemical S-P method was employed to detect the expression of ER-β,MEK-2 and p-ERK in eutopic and ectopic endometrium of patients with EMs(46 cases) and normal controls(40 cases).Results: The expression level of ER-β,MEK-2 and p-ERK in the ectopic and eutopic endometrium of patients were significantly higher than those in control group(P<0.01),and the expression level in ectopic endometrium was higher than those in eutopic endometrium(P<0.01).There were positive correlation between ER-β,MEK-2 and p-ERK in the ectopic endometrium,eutopic endometrium and the normal controls(r=0.628,P<0.01;r=0.559,P<0.01).Conclusion: ER-β,MEK-2 and p-ERK are abnormally overexpressed in endometriosis,and they are positive correlation.MAPK signaling pathways,which is related with estrogen receptor ER-beta,may play an important role in the pathophysiology of EMs.