| 二氟甲基鸟氨酸对T淋巴瘤Jurkat细胞生长的影响 |
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| 引用本文： | 任玉珊,韩钰,曹春雨,王艳林.二氟甲基鸟氨酸对T淋巴瘤Jurkat细胞生长的影响[J].第三军医大学学报,2010,32(6). |
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| 作者姓名： | 任玉珊 韩钰 曹春雨 王艳林 |
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| 作者单位： | 宜昌,三峡大学医学院分子生物学研究所,湖北,443002 |
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| 基金项目： | Supported by the National Natural Science Foundation of China(30772590);;the Key Project of Science Foundation of Department of Education of Hubei Province(D200713003) |
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| 摘 要： | 目的研究二氟甲基鸟氨酸(difluromethylornithine,DFMO)对人T淋巴瘤Jurkat细胞的影响及其作用机制,为探讨DFMO能否用于治疗人白血病提供实验依据。方法DFMO(0～10mmol/L)处理人T淋巴瘤Jurkat细胞24～72h后,MTS法检测细胞的存活率,化学分析法检测精胺氧化酶(spermine oxidase,SMO)和乙酰多胺氧化酶(acetylpolyamine oxidase,APAO)活性,DNA片段化分析检测细胞凋亡,荧光染料法检测细胞线粒体膜电位变化,Western blot法检测细胞内Bax含量,分光光度法检测caspase-3酶活性。结果DFMO处理细胞能显著抑制Jurkat细胞的生长(P<0.01),抑制率随药物浓度的增加和作用时间的延长而加大,DFMO浓度为10mmol/L时,24h抑制率为33%,48h抑制率为38%,72h抑制率为49%。DFMO处理可导致Jurkat细胞DNA片段化,促进Bax由细胞质向线粒体转移,细胞内caspase-3活性增加(增加46%,P<0.05),并伴有线粒体膜电位的显著降低,DFMO浓液为2和5mmol/L时,分别降低29...
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| 关 键 词： | 二氟甲基鸟氨酸 T淋巴细胞 Jurkat细胞 细胞凋亡 生长抑制物 |
Difluoromethylornithine suppresses growth and improves apoptosis of human leukemia Jurkat cells |
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| Ren Yushan,Han Yu,Cao Chunyu,Wang Yanlin.Difluoromethylornithine suppresses growth and improves apoptosis of human leukemia Jurkat cells[J].Acta Academiae Medicinae Militaris Tertiae,2010,32(6). |
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| Authors: | Ren Yushan Han Yu Cao Chunyu Wang Yanlin |
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| Affiliation: | Institute of Molecular Biology;Medical College of Three Gorges University;Yichang;Hubei Province;443002;China |
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| Abstract: | Objective To investigate whether difluromethylornithine (DFMO) can be used in the treatment of human leukemia. Methods The cell proliferation was detected by MTS 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)] assay after treatment of human lymphocyte Jurkat cells by DFMO (0 to 10 mmol/L) for 24 to 72 h. Enzyme activity of spermine oxidase (SMO) and acetylpolyamine oxidase (PAO) was determined by chemiluminesence assay. DNA fragmentation assay was used to evaluate... |
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| Keywords: | difluromethylornithine T-Lymphocytes Jurkat cells apoptosis growth inhibitors |
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