nm23-H1基因转染对人胆管癌细胞系QBC939体外浸润能力的影响

目的：探讨nm23-H1基因转染对人胆管癌细胞系QBC939体外浸润能力的影响。方法：将含有全长nm23-H1 cDNA的真核表达载体通过脂腩体法转染人胆管癌细胞系。结果：转染成功的QBC939细胞，其nm23-Hl基因的mRNA、蛋白表达明显增加，转染nm23-H1基因的胆管癌细胞体外浸润能力下降，穿越matrigel的细胞数明显低于亲本QBC939细胞，代表浸润能力的IV型胶原酶（MMP-9）分泌量下降。结论：nm23-Hl基因可以抑制胆管癌细胞的体外浸润能力。

Effects of nm23-H1 gene transfection on in vitro invasion potential of human cholangiocarcinoma cell line QBC939

Objective To explore the role of nm23 H1 gene overexpression in invasion potential of human cholangiocarcinoma cell line for its potential application in gene therapy for cancer. Methods A mammalian expression vector containing nm23 H1 cDNA was transfected into human cholangiocarcinoma cell line QBC 939 . Northern blotting and immunohistochemistry were used to analyze the expression levels of nm23 H1 and MMP 9, and in vitro invasion potentials the tumor cells before and after the gene transfection. Results After transfection, the nm23 H1 mRNA and protein expression was up regulated significantly. The ability of in vitro invasion through matrigel was remarkably decreased. Immunohistochemical staining showed the expression of MMP 9 were decreased after transfection. Conclusion Up regulation of nm23 H1 in cholangiocarcinoma cell line suppresses its ability of in vitro invasion and reduces the expression of MMP 9 in it.