异种脱蛋白骨的生物相容性研究

目的 评价制备的异种脱蛋白骨植入体内的生物相容性,为临床应用提供实验依据.方法 对猪肋骨进行一系列理化处理后,制成10 mm长脱蛋白骨及浸提液.然后采用标准的毒理学实验方法进行急性、亚急性毒理实验、溶血实验、热源实验、皮内注射实验、肌肉埋植实验及细胞毒性实验.结果 异种脱蛋白骨的毒性程度为无毒,溶血率＜5%,无热源性,对皮肤无刺激,肌肉内埋植后无明显炎症反应,对细胞无明显毒性作用,细胞的毒性均为0级.结论 异种脱蛋白骨具有良好的生物相容性,可满足作为组织工程骨支架材料生物相容性的要求.

On biocompatibility of heterogeneous deproteinized bone as scaffold material

Objective To evaluate the biocompatibility of manufactured heterogeneous deproteinized bone and to provide experimental basis for clinical application. Methods Ten-millimeter long deproteinized bone and leaching liquor were made with a series of physical and chemical method from pig ribs, then were evaluated through acute and subacute toxicity test, hemolysis test, pyrogen test, intracutaneous test, intramuscle implant test and cytotoxicity test. Results No obvious toxicity, hemolysis, pyrogenic characteristics, skin irritation, inflammatory reaction after intramuscle implantation and cytotoxicity were observed. Conclusion The heterogeneous deproteinized bone is of good biological safety and meets all the demand of biocompatibility as scaffold material of tissue engineering bone.