HDV/HBV体外感染原代培养人胎肝细胞的实验研究

目的：建立ＨＤＶ／ＨＢＶ感染人胎肝细胞体外培养系统。方法：利用ＨＤＶ／ＨＢＶ阳性血清同时感染体外２的人胎肝细胞；应用ＥＬＩＳＡ、免疫组化法、原位杂交法和斑点法检测上清液和细胞中ＨＢｓＡｇ、ＨＤＡｇ、ＨＢＶＤＮＡ和ＨＤＶｃＤＮＡ。结果：上清液和细胞中ＨＢｓＡｇ、ＨＤＡｇ、ＨＢＶＤＮＡ和ＨＤＶｃＤＮＡ在感染后第２天至第１６天均可测出，其中上清液中ＨＢｓＡｇ、ＨＤＡｇ以感染后第４天至第１２天达高峰，结论

In vitro infection of HDV/HBV in primary human fetal hepatocytes

Objective: To establish an in vitro culture system of HDV/HBV infected primary human fetal hepatocytes Methods: The cultured primary human fetal heptocytes were simultaneously infected with HDV/HBV positive serum in vitro HDAg, HBsAg, HDV cDNA and HBV DNA were detected with ELISA, immunohistochemistry, in situ hybridization and dot blot hybridization in supernatant and cells Results: HDAg, HBsAg, HDV cDNA and HBV DNA were detected from the 2nd day to the 16th day after infection in supernatant and cells Secretion of HDAg and HBsAg reached peaks from the 4th day to the 12th day after the infection in the supernatant Conclusion: Primary human fetal hepatocytes are competent for infection with HDV/HBV HDV and HBV can steadily replicate and express in HDV/HBV infected human fetal hepatocytes and the replication and expression can at least last for 12 days