| PI3K/Akt通路在硫化氢后处理保护心肌细胞缺氧复氧损伤中的作用 |
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| 引用本文： | 李钢,杨双强.PI3K/Akt通路在硫化氢后处理保护心肌细胞缺氧复氧损伤中的作用[J].第三军医大学学报,2011,33(17). |
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| 作者姓名： | 李钢 杨双强 |
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| 作者单位： | 重庆医科大学附属第一医院胸心外科,重庆,400016 |
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| 摘 要： | 目的探讨硫化氢后处理对原代培养的新生大鼠心肌细胞缺氧复氧损伤的保护作用及可能机制。方法以硫氢化钠(NaHS)作为外源性硫化氢供体。原代培养新生大鼠的心肌细胞随机分为5组:①正常对照组;②缺氧复氧组(hypoxia-reoxygenation,HR组);③二甲基亚砜组(dimethyl sulfoxide,DMSO组);④硫化氢后处理组(hydrogen sulfide post-con-ditioning,H2S组);⑤LY294002+H2S后处理组(LY+H2S组)。分别于缺氧前和复氧2 h检测各组的心肌细胞存活率、培养液中CK和LDH的活性;复氧末,用流式细胞学技术检测各组心肌细胞凋亡情况;Western blot检测HIF-1α、p-Akt与Akt蛋白的表达情况。结果通过比较可知,复氧2 h时,H2S组心肌细胞存活率较HR组升高显著(71.55±1.46)%vs(62.03±1.29)%,P<0.01],CK、LDH活性以及心肌细胞凋亡率明显降低(244.99±21.38)U/L vs(329.53±26.14)U/L,(371.64±18.62)U/L vs(427.69±13.25)U/L,(...
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| 关 键 词： | 硫化氢 心肌缺血再灌注损伤 缺氧诱导因子-1α 凋亡 PI3K/Akt |
Role of PI3K/Akt pathway in protection of cardiomyocytes against hypoxia-reoxygenation injury induced by hydrogen sulfide post-conditioning |
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| Li Gang,Yang Shuangqiang.Role of PI3K/Akt pathway in protection of cardiomyocytes against hypoxia-reoxygenation injury induced by hydrogen sulfide post-conditioning[J].Acta Academiae Medicinae Militaris Tertiae,2011,33(17). |
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| Authors: | Li Gang Yang Shuangqiang |
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| Affiliation: | Li Gang,Yang Shuangqiang(Department of Cardiothoracic Surgery,First Affiliated Hospital of Chongqing Medical University,Chongqing,400016,China) |
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| Abstract: | ObjectiveTo study the protective effect of hydrogen sulfide(H2S) post-conditioning on hypoxia-reoxygenation injury in primary cultured cardiomyocytes from neonatal rats and its possible mechanism.Methods Sodium hydrosulfide(NaHS) was used as an exogenous donor of H2S.Primary cultured cardiomyocytes from neonatal rats were randomly divided into control group,hypoxia-reoxygenation(HR) group,dimethyl sulfoxide(DMSO) group,H2S post-conditioning group,and LY294002+H2S post-conditioning group.Survival rate of car... |
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| Keywords: | hydrogen sulfide myocardial ischemia-reperfusion injury hypoxia-inducible factor-1alpha apoptosis PI3K/Akt |
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