cDNA微阵列和组织微阵列对卵巢上皮性肿瘤基因的表达

目的]结合cDNA微阵列和RNA与冰冻组织微阵列原位杂交的方法以寻找新的特异性卵巢癌候选癌基因,为卵巢癌的早期诊断和治疗提供理论依据.方法]利用cDNA微阵列筛选在所有3种上皮性卵巢肿瘤中(卵巢浆液性交界性肿瘤、卵巢浆液性腺癌和卵巢子宫内膜样腺癌)显示有意义表达的基因,由RNA与冰冻组织微阵列原位杂交证实其结果.结果]28个基因在＞70%卵巢肿瘤中显示出高表达,18个基因在＞70%卵巢肿瘤中低表达;干扰素诱导的转膜蛋白1(ⅡTP1)被进一步用于RNA与冰冻组织微阵列原位杂交研究,其结果与cDNA微阵列研究结果相符合.结论]将cDNA微阵列和RNA与冰冻组织微阵列原位杂交相结合是寻找卵巢癌候选癌基因的可行方法,研究中显示有意义表达的基因有可能作为新的上皮性卵巢癌候选癌基因.

Gene Expression Profiles to Epithelial Ovarian Tumors by Using cDNA Microarray and Tissue Microarrays

Combining cDNA microarray with RNA i n situ hybridization on frozen tissue microarray to identify novel candidate oncogenes and provide possible theoretical basis for early diagnosis and treatment of ovarian c ancer.cDNA microarrays were used to seek significantly expressed genes in 3t ypes of ovarian tumors(serous borderline ovarian tumors,s erous ovarian cancers,and endometrioid ovarian carcinoma s).RNA in situ hybridization on frozen tissue microarray was used to confirm the fi nding from cDNA microarrays.In the study of cDNA microarray,28genes were over-expr essed and 18genes were under-expressed in >70%ovarian tumors.Interferon induced transme mbrane protein 1(IITP1)was further validated by RNA in situ hybridization on frozen tissue microarray.Conclusion]The methods through combining cDNA microarray with RNA in situ hybridiz ation on frozen tissue microarray,i s an ideal choice for identifying novel oncogenes.The ge nes identified in this study might be the new candidate oncogenes of ovarian cancer.