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变温层积对解除滇重楼种子休眠及其内源激素变化的研究
引用本文:陈疏影,尹品训,杨艳琼,王 荔,叶 玉,沈 阳.变温层积对解除滇重楼种子休眠及其内源激素变化的研究[J].医学教育探索,2011,42(4):793-795.
作者姓名:陈疏影  尹品训  杨艳琼  王 荔  叶 玉  沈 阳
作者单位:云南农业大学农学与生物技术学院,云南 昆明 650201
基金项目:国家自然科学基金资助项目(30860367)
摘    要:目的 研究滇重楼种子破休眠的方法及其内源激素水平变化规律。方法 以变温层积处理的滇重楼种子为材料,利用高效液相色谱法(HPLC)测定种胚发育过程中植物激素赤霉素(GA)、吲哚乙酸(IAA)、脱落酸(ABA)量的变化与种子胚率的关系。结果 变温层积处理可在3个月内打破滇重楼种子休眠,其GA量显著升高,ABA量显著下降。结论 内源激素ABA和GA在滇重楼种子休眠与萌发过程中起着重要的作用。

关 键 词:滇重楼种子  变温层积处理  解除休眠  种胚发育  内源激素

Rule of breaking Paris polyphylla var. yunnanensis seed dormancy under fluctuating temperature stratification and content changes of endogenous hormone
CHEN Shu-ying,YIN Pin-xun,YANG Yan-qiong,WANG Li,YE Yu,SHEN Yang.Rule of breaking Paris polyphylla var. yunnanensis seed dormancy under fluctuating temperature stratification and content changes of endogenous hormone[J].Researches in Medical Education,2011,42(4):793-795.
Authors:CHEN Shu-ying  YIN Pin-xun  YANG Yan-qiong  WANG Li  YE Yu  SHEN Yang
Affiliation:College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China
Abstract:Objective To study the rule of breaking seed dormancy and content changes of endogenous hormone of the Paris polyphylla var. Yunnanensis seeds. Methods The seeds were treated under fluctuating temperature, and the content of endo-hormone, such as GA, IAA, and ABA, was mensurated by HPLC. Results During the first three months, the stratification under fluctuating temperature could break the seed dormancy. The content of GA rose obviously and the content of ABA reduced. Conclusion It is suggested that ABA and GA have significant effect on the dormancy and germination of P. polyphylla var. yunnanensis seed.
Keywords:Paris polyphylla var   yunnanensis(Franch  )Hand  -Mazz   seed  fluctuating temperature stratification  break dormancy  embryo development  endogenous hormone
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