大孔吸附树脂分离肿节风中总黄酮的研究

目的考察11种大孔吸附树脂对肿节风总黄酮的吸附分离性能。方法采用静态吸附分离法确定适合的大孔吸附树脂;采用动态吸附分离法确定分离条件。以总黄酮吸附量、总黄酮质量分数和回收率为考察指标,采用紫外分光光度法测定总黄酮。结果HPD 400大孔吸附树脂对肿节风总黄酮有良好的吸附分离性能,其分离肿节风总黄酮的工艺条件为:肿节风总黄酮上样质量浓度为10 m g/mL,肿节风总黄酮最大吸附量为9.5 m g/mL,吸附体积流量为2.5 mL/m in,洗脱剂为70%乙醇,洗脱剂用量为3倍柱体积,树脂可重复使用3次。结论采用HPD 400大孔吸附树脂吸附分离肿节风总黄酮简便有效,总黄酮回收率为85%左右。

Separation of total flavone in Sarcandra glabra by macroporous adsorption resins

Objective To set up the technology for adsorbing and separating total flavone in Sarcandra glabra with macroporous adsorption resin,11 types of macroporous adsorption resins were investigated.Methods The static adsorption and separation were used in investigation of macroporous adsorption resin;the dynamic adsorption and separation were used in studying the condition of adsorption and separation.The total flavone adsorption capacity,total flavone content,and total flavone recovery rate were used as the evaluation criteria.The UV spectrophotometric method was used in the determination of total flavone content.Results The results showed that among 11 types of macroporous adsorption resins,the HPD400 was the best for adsorbing and separating the total flavone in S.glabra in the following technological condition: the concentration of S.glabra sample extract was 10 mg/mL;the maximum adsorbing capacity for total flavone in S.glabra was 9.5 mg/mL;the current velocity was 2.5 mL/min;the eluting reagent was 70% ethanol(three times as the volume of the resin);and the HPD400 resin could be used three times,repeatedly.Conclusion It is a simple and efficient to separate the total flavone in S.glabra under the technological conditions,and a total flavone recovery rate is about 85%.