JNK抑制肽XG-102在大鼠脑出血中的保护作用

目的 研究c-Jun氨基末端激酶（JNK）抑制肽XG-102在大鼠脑出血中的作用.方法 51只雄性SD大鼠被随机分成假手术组、脑出血组和XG-102治疗组,脑出血组和XG-102治疗组大鼠采用自体动脉血脑内注射构建脑出血模型,XG-102治疗组大鼠于脑出血后3h静脉注射100 μg/kg XG-102,采用改良神经功能缺损程度评分（mNSS）检测各组大鼠神经功能,干湿重法测定大鼠脑组织含水量的变化,免疫印迹检测大鼠血肿周围水通道蛋白（AQP4）和p-c-Jun的表达.结果 与脑出血组相比,在大鼠脑出血后1天XG-102治疗组神经功能评分降低（P＜0.05）,脑出血后2天脑水肿减轻（P＜0.01）,脑出血后2天p-c-Jun的表达减少、AQP4的表达增加（P＜0.01）.结论 XG-102在大鼠脑出血后早期即可减轻脑水肿,改善神经功能缺损,发挥神经保护作用。

The JNK inhibitor XG-102 protects against intracerebral hemorrhage

Objective To investigate the protective effect of an JNK inhibitor XG-102 on intracerebral hemorrhage （ICH）.Methods Fifty-one male SD rats were randomized into a sham-operated group,an ICH group and an XG-102 treatment group.ICH was induced by injection of 50 μl of autologous arterial blood into rat brains.The rats in the XG-102 treatment group were intravenously injected with 100 μg/kg of XG-102 three hours after ICH.Modified neurological severity scores （mNSS） were assess to measure the neural function of each rats.The changes of brain water content （BWC） were measured by the wet and dry weight method.The expression of phospho-c-Jun and AQP4 in the peripheral region of hematoma were examined by Western blot.Results Compared with the ICH group,the XG-102 treatment group presented significantly improved neurological outcome 1 day after ICH （P 〈 0.05）.Brain water content was also significantly decreased 2 days later （P 〈 0.01）.The administration of XG-102 suppressed the level of phospho -c-Jun and increased the level of AQP4 in the peripheral region of hematoma 2 days after ICH （P 〈 0.01）.Conclusion XG-102 improves neurological function,attenuates cerebral edema,and provides strong neuroprotection at a nearly stage after ICH.