| CD44在不同肿瘤细胞表面阳性表达率的实验研究 |
| |
| 引用本文： | 王珏,沈雁,张俊玲,涂家生.CD44在不同肿瘤细胞表面阳性表达率的实验研究[J].山东医药工业,2014(2):63-67. |
| |
| 作者姓名： | 王珏 沈雁 张俊玲 涂家生 |
| |
| 作者单位： | [1]中国药科大学药学院药剂学教研室,江苏南京210009 [2]江苏省苏北人民医院肿瘤科,江苏扬州225001 |
| |
| 基金项目： | 中央高校基本科研业务费专项基金资助项目（No.JKPZ2013006）;国家自然科学基金资助项目（No.81072588,81201182） |
| |
| 摘 要： | 目的 检测CD44在肿瘤细胞表面的阳性表达率,为以CD44为靶点的肿瘤靶向治疗提供新的理论依据.方法 采用流式细胞术检测HCT116人结肠癌细胞,SGC-7901、MGC-803胃癌细胞,A549人肺腺癌细胞,HepG2人肝癌细胞,CNE-2人鼻咽癌细胞,U87MG恶性胶质瘤等肿瘤细胞表面CD44表达情况.结果 各肿瘤细胞表面均表达CD44.其中胃癌SGC-7901、MGC-803、肝癌HepG2、鼻咽癌CNE-2细胞表面CD44细胞的阳性率分别为17.90％±1.67％、18.36％±9.27％、24.61％±4.0％和19.03％ ±1.52％,呈高表达状态.结论 SGC-7901、MGC-803、HepG2、CNE-2细胞与HCT116、A549和U87MG细胞表面CD44阳性表达率相比有显著性差异（P＜0.05）.
|
| 关 键 词： | CD44 肿瘤靶向 流式细胞术 阳性表达率 |
Analysis on the express of surface adhesion molecule CD44 in different tumor cells |
| |
| Authors: | WANG Jue SHEN Yan ZHANG Jun-ling TU Jia-sheng |
| |
| Affiliation: | 1. Department of Pharmacy, China Pharmaceutical University, Nanjing 210009, China ;2. Department of Oncology, Subei People＇s Hospital of Jiangsu Province, Yangzhou 225001, China ) |
| |
| Abstract: | Objective To detect the expressions of surface adhesion lnolectde CD44 in tulnor cells in order to provide a new theoretical reference for CD44 tumor targeted therapy. Methods The expressions of CD44 of HCT116, SGC - 7901, MGC -803,A549, HepG2,CNE -2 and U87MG cells were determined by flow cytometry. Results CD44 was all expressed on these tmnor cells. CD44 was over expressed on SGC -7901 ,MGC - 803,HepG2 and CNE -2 and the positive expression rate were17. 90% ± 1. 67% ,18. 36% ± 9. 27% ,24. 61% ±4. 0% and 19. 03%±1.52%,respeetively. Conelusion There was signillcant difference between the positive expression rate of SGC - 7901, MGC - 803, HepG2, CNE - 2 and HCT116, A549,U87MG（ P 〈0. 05）. |
| |
| Keywords: | CD44 Tuxnor targeted Flow cytometry Positive expression rate |
| 本文献已被 维普 等数据库收录！ |
|
