基于有效基准特征图谱质量表征模式的中药紫苏叶质量评价研究

目的建立基于紫苏叶有效基准特征图谱的质量表征模式、方法与质量评价应用,以精准、有效地评价紫苏叶饮片质量。方法以确定具有调节血脂活性的紫苏叶饮片作为饮片基准,以有效特征指标性成分作为质量表征基准点,采用HPLC-PDA法建立有效基准特征图谱,并以有效基准特征图谱的质量表征信息作为评价紫苏叶饮片质量的标尺,同时对其准确性进行佐证。结果以野黄芩苷为基准点(保留时间:30.342 min;饮片含量:0.573 4%;检测溶液浓度:0.057 34 g/L),表征紫苏叶有效饮片基准(S1)特征图谱质量信息:14个特征峰保留时间、峰面积、相对保留时间、相对峰面积,6个特征指标性成分(咖啡酸、木犀草素-7-氧-二葡萄糖醛酸苷、芹菜素-7-氧-二葡萄糖醛酸苷、野黄芩苷、迷迭香酸、齐墩果酸)含量、相对含量及含量换算系数,其涵盖了酚酸类、黄酮类及三萜类药学架构主要化学类型。同时以野黄芩苷对照品模拟基准点(保留时间:29.759 min;检测溶液浓度:0.057 3 g/L)表征质量信息,二者相吻合佐证了检测方法可行性及检测结果的准确性。14批待评价紫苏叶饮片色谱图中均含有此14个特征峰,6个特征指标性成分换算含量与实测含量相吻合;以有效基准质量表征信息为标尺评价14批紫苏叶饮片质量,综合得出编号S8、S7、S12、S9、S13饮片优良度居前。结论建立的基于紫苏叶有效基准特征图谱的质量表征模式、方法,可简易、准确、整体地表征紫苏叶饮片质量;构建的紫苏叶质量评价模式可有效、精准地评价紫苏叶饮片质量。

Quality evaluation of Perillae folium based on representation model of effective characteristic spectrum of reference

Objective To establish the quality representation model, analysis method and quality evaluation based on the effective characteristic spectrum of reference of perillae folium, so as to evaluate the quality of perillae folium effectively and accurately. Methods To determine the perillae folium which has the effect of regulating blood lipid as the standard reference pieces. Taking effective characteristic index component as quality reference point. Using HPLC-PDA method to establish effective characteristic spectrum of reference, and obtained quality characterization information was used as the standard to evaluate the quality of perillae folium, and to prove the accuracy. Results Scutellarin, as the reference point,(retention time: 30.342 min;content: 0.573 4%;concentration: 0.057 34 g/L), represented the quality information of characteristic spectrum of benchmark(S1) which was effective:14 characteristic peak retention time, peak area, relative retention time, relative peak area, six characteristic index components content, relative content and content conversion coefficient(caffeicacid,luteolin-7-O-glucuronide, apigenin-7-O-glucuronide, scutellarin, rosmarinic acid, oleanolic acid), which covers phenolic acids, flavonoids and triterpenoids. And reference substance of scutellarin, as the simulative datum point(rretention time: 29.759 min;concentration: 0.057 3 g/L), represented the quality information. The two results were consistent, and verified the feasibility of the test method and the accuracy of the test results.14 characteristic peaks appeared on the chromatograms of 14 batches of perillae folium and the conversion content of six characteristic index components was consistent with the measured content. Using the effective quality representation of reference information to evaluate the quality of 14 batches of perillae folium, and the quality of S8,S7,S12,S9,S13 were judged to be better. Conclusion The established quality representation model and analysis method based on the effective characteristic spectrum of reference of perillae folium can be used represent the quality of perillae folium simply, accurately and integrally. The established quality analysis model can be used to analyze the quality of perillae folium.