| 定量PCR与EIA法检测血液透析患者HCV比较 |
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| 引用本文： | 林潮双,韦嘉,卢建溪,彭晖,高志良.定量PCR与EIA法检测血液透析患者HCV比较[J].热带医学杂志,2001,1(1):45-47. |
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| 作者姓名： | 林潮双 韦嘉 卢建溪 彭晖 高志良 |
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| 作者单位： | 中山医科大学第三附属医院传染科,广州,510630 |
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| 基金项目： | 卫生部部属重点院校临床重点项目资助（课
题编号：97040232） |
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| 摘 要： | 目的 探讨血液透析患丙型肝炎病毒（HCV）的检测方法。方法 对79例尿毒症长期血液透析患，采用荧光定量PCR法测定血清HCV-RNA水平，及第二代酶免疫试验（EIA）检测丙型肝炎病毒抗体（抗-HCV）。结果 血液透析患抗-HCV阳性率22.8%(18/79)，荧光定量PCR法HCV-RNA检出率39.2%(31/79）。在抗HCV阴性的患中，HCV-RNA的检出率为42.6%(26/61)。两种方法检测结果的符合率为50.6%(40/79)。结论 荧光定量PCR技术可弥补EIA检测的不足，在抗-HCV阴性的血液透析患中检测HCVRNA具有重要意义。
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| 关 键 词： | HCV 比较 荧光定量PCR 血液透析 丙型肝炎病毒RNA |
| 文章编号： | 0254-900X(2001)-0045-03 |
Study on HCV in patients of haemodialysis by amplisensor quantitative PCR and EIA |
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| Lin Chaoshuang,WEI Jia,Lu Jianxi,Pen Hui,GAO Zhiliang.Study on HCV in patients of haemodialysis by amplisensor quantitative PCR and EIA[J].Journal Of Tropical Medicine,2001,1(1):45-47. |
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| Authors: | Lin Chaoshuang WEI Jia Lu Jianxi Pen Hui GAO Zhiliang |
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| Affiliation: | Lin Chaoshuang,Wei Jia,Lu Jianxi,Pen Hui,Gao Zhiliang Department of Infectious Diseases,The Third Affiliated Hospital,Sun Yat- Sen University of Medical Sciences,Guangzhou 510630 |
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| Abstract: | Objective The aim of the paper is to study the detection of HCV in patients of haemodialysis. Methods Serum from 79 haemodialysis patients were tested for hepatitis C viral RNA titers by amplisensor quantitative PCR and Anti-HCV by secondary enzyme immune assay (EIA-2). Results Positive rate of anti-HCV in haemodialysis patients was 22. 8%. Positive rate of HCV - RNA by amplisensor quantitative PCR was 39. 2%. Of anti-HCV negative patients, positive rate of HCV RNA by amplisensor quantitative PCR was 42. 6% . The coincidence rate of the two methods is 50. 6% Conclusions Amplisensor quantitative PCR could make up weakness of EIA . It is necessary to detect HCV RNA in haemodialysis patients of anti-HCV negative. |
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| Keywords: | amplisensor quantitative PCR haemodialysis HCV-RNA |
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