Epstein-Barr病毒LMP1-exonl反义表达载体对NPC细胞生长的抑制作用

目的　研究EB病毒LMP1反义基因对人鼻咽癌CNE2细胞恶性表型的逆转作用。方法　用反义LMP1DNA的真核表达载体anti—pcDNA3．1—LMP1转染人鼻咽癌细胞CNE2，G418筛选抗性克隆;Western-blot法检测转染前、后瘤细胞的LMP1表达；用MTT法、细胞生长曲线和软琼脂集落形成能力实验观察转染前后细胞体外生长增殖特性。结果建立反义CNE2细胞克隆；转染后LMP1蛋白表达降低；转染后细胞活力和生长速度均有下降，集落形成能力显降低。结论　将反义LMP1真核表达载体导入细胞能成功抑制NPC细胞的生长，逆转其恶性表型。本研究还为鼻咽癌的基因治疗提供了实验依据。

Inhibition of NPC Cell-growth by Antisense EB Virus LMP1-exonl Gene

Objective To study the reverse effect on the malignant phenotype of human NPC cells CNE2 transfected with EBV-LMP1 antisense gene.Methods The anti-pcDNA3.1-LMP1 gene were transferred into human NPC cells CNE2.The cell colonies resistant to G418 were selected.The LMP1 expression of CNE2 cells before or after transfection was detected by Western blot.The cellular proliferation capacity was detected by MTT assay.Cell growth curve and the colony formation of the cells in soft agar medium.Results The expression of LMP1,the proliferative capacity of antisense LMP1 gene transfected CNE2 cells in vitro were obviously decreased. The capality of clony formation in soft agar was markedly dropped.Conclusion The antisense LMP1 gene can suppress the growth of CNE2 cells,and reverse their malignant phenotype.This study provided experimental basis for the gene therapy of the nasopharyngeal carcinoma.