高浓度软脂酸体外抑制人外周血内皮祖细胞的增殖

目的 观察软脂酸对体外培养的人外周血内皮祖细胞(EPCs)增殖的影响.方法 采用密度梯度离心法分离培养人外周血单个核细胞,经FITC标记荆豆凝集素I(FITC-UEA-I)和Dil标记的乙酰化低密度脂蛋白(Dil-acLDL)双染色鉴定为正在分化的EPCs,并进一步通过流式细胞仪检测其表面标志CD34、CD133和VEGFR2.分别以不同浓度(0、50、100、200、400、800 μmol/L)软脂酸溶液作用48 h,以及400μmol/L软脂酸溶液作用不同时间(0、12、24、36、48和60 h)后,CCK-8法观察EPCs增殖的情况.结果 与对照组比较,400 μmol/L组及800 μmol/L软脂酸组均抑制了EPCs的增殖功能(P<0.05),在400μmol/L时最为显著;且400μmol/L软脂酸对EPCs增殖抑制作用随时问延长逐渐增强.48 h达到高峰(增殖抑制率为58.59%(P<0.05).结论 高浓度软脂酸体外能显著抑制外周血内皮祖细胞的增殖.

High-concentration palmitic acid inhibits the proliferation of peripheral blood-derived human endothelial progenitor cells in vitro

Objective To study the effects of palmitic acid(PA) on the proliferation of peripheral blood-derived endothelial progenitor cells(EPCs) in vitro.Methods The mononuclear cells(MNCs) were isolated from the peripheral blood by Ficoll density-gradient centrifugation.The isolated EPCs were characterized by Di-LDI uptake and FITC-lectin binding assay using laser confocal microscope, and further identified by detection of CD34, CD133 and VEGFR2 expression using flow cytometry.The cultured EPCs were incubated in th...