大鼠肾移植后移植肾内树突状细胞的早期观察

目的 观察大鼠肾移植后早期移植肾内树突状细胞(DC)的变化规律,藉此探讨排斥反应的防治.方法 受体Wistar大鼠30只,按5只/组随机分为6组,35只SD大鼠作为供体,其中5只供肾作为对照组,不行受体手术.其余均行同种异体原位肾移植术,并分别于开放供肾循环后1、6、12、24、48、72 h切取移植肾,行石蜡包埋切片,抗S-100蛋白免疫组化染色和HE染色.观察移植肾病理学改变及肾小球内DC数量变化.结果 各组切片均未见肾小管肾炎、动脉内膜炎等AR表现.对照组及术后1 h组肾小球内DC数量基本为零;此后逐渐增加;24 h组达到顶峰,其均数与其他各组相比,差异显著(Pmax=0.038);随后DC数量缓慢下降.结论 大鼠肾移植术后72 h内,供肾肾小球DC数量呈单峰曲线变化:受体DC不断迁入、迁出供肾是其变化的主要原因;揭示移植肾内DC变化规律有助于进一步完善免疫抑制剂及抗炎药物的使用方案.

Change of renal graft dendritic cells in the early stage following transplantation: a dynamic observation in rats

Objective To observe the dynamic changes of dendritic cells(DCs)in the renal graft of rats within 72 h after renal transplantation.Methods Using SD rats as the donors and Wistar rats as the recipients,renal transplantation was performed in 30 pairs of rats,with another 5 donor kidneys that were not transplanted serving as the sham operation group.The transplanted kidneys were harvested at 1,6,12,24,48 and 72 h after recovery of blood circulation,paraffin-embedded and sectioned,followed by HE staining and immunohistochemical staining for S-100 protein for DC identification.The pathological changes and the DC density per glomerulus in the renal graft were observed with optical microscope.Results No signs of acute rejection were found in these sections.Few DCs were observed in the sham operation group and in the renal graft 1 h after transplantation.The number of DCs in the renal graft increased with time and reached the maximum 24 h after transplantation followed by gradual decrease.Conclusions Within 72 h after renal transplantation,the number of DCs in the graft varies following a curve with a single peak.Increased DC density in the graft may result from recipient DC migration into the graft,and accordingly,decreased recipient DC migration results in decrease of DC density in the graft.The pattern of DC number variation in the graft can be helpful to further improve the therapy against graft rejection.