失笑散对实验性心肌缺血大鼠血液流变学的影响

目的:观察失笑散颗粒对心肌缺血大鼠血液流变学的影响。方法:60只雄性SD大鼠随机平均分成空白对照组,模型组,失笑散颗粒低剂量组、中剂量组、高剂量组,阳性药物对照组。空白对照组和模型对照组给予等量生理盐水,其余各组分别给予相应药物,均采用灌胃方式,连续7 d,从第3日起,除正常对照组外,其余各组均于给药后1 h皮下注射异丙肾上腺素5 mg·(kg·d)~(-1),连续5 d,时间间隔为24 h;正常对照组给予等量生理盐水皮下注射,记录给药前及第5次注射异丙肾上腺素后心电图。末次注射后2 h,处死动物,取血制备血清,用全自动生化检测仪测定心肌酶;处死后立即取出心脏,制备心肌匀浆,试剂盒法测定超氧化物歧化酶(superoxide dismutase,SOD)和丙二醛(malonaldehyde,MDA)。结果:模型组与空白对照组相比,实验大鼠心肌缺血阳性率明显升高,全血黏度、血浆黏度、红细胞压积均升高,血沉降低,天门冬氨酸氨基转移酶(aspartate amino transferase,AST)、乳酸脱氢酶(lactic dehydrogenase,LDH)、磷酸肌酸激酶(creatine phosphate kinase,CPK)、脑利钠肽(brain natriuretic peptide,BNP)、肌钙蛋白T(troponin T,Tn T)、MDA均升高(P0.05),SOD降低(P0.05);失笑散颗粒高剂量组与模型组相比,心肌缺血阳性率明显降低,全血黏度、血浆黏度、红细胞压积均降低,血沉升高,AST、LDH、CPK、BNP、Tn T、MDA均降低(P0.05),SOD升高(P0.05);阳性药物对照组与模型组相比,心肌缺血阳性率降低,全血黏度、血浆黏度、红细胞压积均升高(P0.05),LDH、CPK均降低(P0.05)。结论:失笑散颗粒能够缓解实验性大鼠心肌缺血症状,且高剂量的失笑散颗粒对心肌缺血的疗效更显著,为失笑散颗粒型的临床应用提供了制剂学基础和药效学依据。

Effect of Shixiao Powder on Hemorheology in Rats with Experimental Myocardial Ischemia

Objective:To observe the effect of Shixiao Powder on hemorheology in rats with myocardial ischemia.Methods:Sixty male SD rats were randomly divided into blank control group,model group,low dose group,middle dose group,high dose group and positive drug control group.The control group and the model control group were given the same amount of normal saline,and the other groups were given the corresponding drugs for 7 days.From the 3rd day,all groups except for the blank control group were injected subcutaneously with isoproterenol (ISO) 5 mg · (kg · d)-1.The injection lasted for 5 days and the time interval was 24 h.The blank control group was given subcutaneous injection of the same amount of normal saline.The electrocardiogram before drug administration and that after the fifth injection of ISO were recorded.2 hours after the last injection,the animals were sacrificed and the serum was collected.The myocardial enzymes were measured by automatic biochemical detector.The hearts were sacrificed immediately after the sacrifice,and the myocardium homogenate was prepared.The superoxide dismutase (SOD) and the Malondialdehyde (MDA) were detected.Results:Compared with that of the blank control group,the positive rate of myocardial ischemia,the whole blood viscosity,the plasma specific viscosity and the hematocrit increased of the experimental group were significantly higher than that of the control group (P ＜ 0.05),and the aspartate amino transferase (AST),lactate dehydrogenase (LDH),creatine phosphate kinase (CPK),brain natriuretic peptide (BNP),brain natriuretic peptide Troponin T (TnT) and MDA of the experimental group significantly increased (P ＜ 0.05).The blood sedimentationratio and SOD decreased (P ＜ 0.05).Compared with that of the model group,the positive rate of myocardial ischemia was significantly lower than that of the model group.The ratio of whole blood viscosity,plasma specific viscosity,hematocrit decreased and erythrocyte sedimentation rate increased;the AST,LDH,CPK,BNP,TnT,MDA decreased (P ＜ 0.05) while SOD increased (P ＜ 0.05).Compared with that of the model group,the positive rate of myocardial ischemia,plasma specific viscosity,plasma specific viscosity and hematocrit increased (P ＜ 0.05),while LDH and CPK decreased (P ＜ 0.05).Conclusion:Shixiao Powder can relieve the symptoms of myocardial ischemia in experimental rats,and the effect of high dose of it on myocardial ischemia is more significant.The study can provide a pharmacodynamics basis for the clinical application of the medicine.