ZD6474对肝癌细胞和乙肝相关肝癌细胞增殖的影响

目的:探讨ZD6474对肝癌细胞株HepG2和乙肝相关肝癌细胞株HepG2.2.15细胞增殖的影响.方法:用WST方法检测ZD6474对细胞增殖的抑制作用;采用流式细胞仪检测ZD6474对细胞周期及凋亡的影响.结果:ZD6474可以显著抑制HepG2和HepG2.2.15细胞增殖,6.4 μmol/L的ZD6474可以抑制(46.86±10.32)％的HepG2和(41.24±7.35)％的HepG2.2.15细胞增殖,其抑制增殖作用随剂量增加而增强(P＜0.05).ZD6474诱导细胞产生G0/G1期阻滞,6μmol/L的ZD6474可诱导71.90％的HepG2和69.90％的HepG2.2.15细胞阻滞于G0/G1期.结论:ZD6474可以抑制HepG2和HepG2.2.15细胞增殖,其机制可能与诱导细胞周期阻滞有关.

Inhibitory Effect of ZD6474 on Human Hepatocellular Carcinoma Cells

Objective: To investigate the inhibitory effects of ZD6474 on human hepatocellular carcinoma HepG2 and hepatitis-B related HepG2.2.15 cells.Methods: WST methods was used to examine the inhibitory effect of ZD6474 on HepG2 and HepG2.2.15 cells.The cell cycle distribution influence of ZD6474 on HepG2 and HepG2.2.15 cells was detected by flow cytometry.Results: ZD6474 significantly inhibited the growth and induced G0/G1 phase arrest of HepG2 and HepG 2.2.15 cells(P<0.05).6.4 μmol/L ZD6474 inhibited the proliferation of(46.86±10.32) % HepG2 cells and(41.24±7.35) % HepG2.2.15 cells.6 μmol/L ZD6474 induced 71.90% HepG2 cells and 69.90% HepG2.2.15 cells accumulated in G0/G1 phase.Conclusion: ZD6474 can inhibit the proliferation of HepG2 and HepG2.2.15 cells.The mechanism may be correlated with ZD6474-induced cell cycle arrest at G0/G1 phase of hepatocellular carcinoma cells.