| 输血传播病毒在原发性肝癌患者中的感染状况研究 |
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| 引用本文： | 姚春斓,周元平,李建国.输血传播病毒在原发性肝癌患者中的感染状况研究[J].广东医学,1999,20(11):833-834. |
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| 作者姓名： | 姚春斓 周元平 李建国 |
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| 作者单位： | 中山医科大学附属第三医院传染科!510630 |
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| 基金项目： | 广东省科委自然科学基金!资助(项目编号 :961 54) |
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| 摘 要： | 目的 了解新型肝炎病毒输血传播病毒（ｔｒａｎｓｆｕｓｉｏｎ ｔｒａｎｓｍｉｔｔｅｄ ｖｉｒｕｓ，ＴＴＶ）在原发性肝癌（ＰＬＣ）患者中的感染状况。方法 在ＴＴＶ长的ＯＲＦ保守区设计引物，建立巢式聚合酴链反应（ＰＣＲ）技术，检测了１５１例原发性肝癌患者血清中ＴＴＶ ＤＮＡ。结果 １５１例原发性肝癌患者中有２８例ＴＴＶ ＤＮＡ阳性，阳性率为１８．５％；而同时检测１２５例正常人血清只３例ＴＴＶ阳性，阳性率
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| 关 键 词： | 原发性肝癌 输血传播病毒 巢式 聚合酶链反应 |
Prevalence of transfusion transmitted virus infection in patients with primary liver cancer |
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| Yao Chunlan,Zhou Yuanping,Li Jianguo,et al..Prevalence of transfusion transmitted virus infection in patients with primary liver cancer[J].Guangdong Medical Journal,1999,20(11):833-834. |
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| Authors: | Yao Chunlan Zhou Yuanping Li Jianguo |
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| Affiliation: | Yao Chunlan,Zhou Yuanping,Li Jianguo,et al. Department of Infectious Diseases,Third Affiliated Hospital,Sun Yat-sen University of Medical Sciences,Guangzhou 510630 |
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| Abstract: | Objective To investigate the prevalence of transfusion transmitted virus (TTV) in patients with primary liver cancer (PLC).Methods A nested polymerase chain reaction (PCR) assay with primers from ORF1 of TTV genome was established to detect TTV DNA in sera of 151 PLC patients and 125 controls.Results 28 of 151 (18 5%) PLC patients and 3 of 125 (2 4%) controls were positive for TTV DNA ( P <0 005).Conclusion Prevalence of TTV DNA in the patients with PLC is significantly higher than that in controls. The nested PCR assay of TTV DNA is a useful technique for studying relationship between TTV infection and PLC. |
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| Keywords: | Primary liver cancer\ Transfusion transmitted virus\ Nested polymerase chain reaction |
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