低剂量辐射对荷瘤小鼠免疫功能的刺激作用

目的：探讨低剂量电离辐射对荷瘤机体免疫功能的影响。方法：采用 Ｃ５７ Ｂ Ｌ／６ Ｊ小鼠右后肢腓肠肌内接种 ｌｅｗ ｉｓ 肺癌细胞做为实验动物模型，在肿瘤接种后 １０ ｄ，给予 ７５ ｍ Ｇｙ Ｘ 射线全身照射，于照射后 １８ ｈ 测定荷瘤小鼠胸腺、脾脏某些免疫学参数的变化，包括胸腺细胞自发增殖力、脾细胞数，脾细胞对 Ｃｏｎ Ａ、 Ｌ Ｐ Ｓ反应性，脾细胞白细胞介素（ Ｉ Ｌ ２）分泌量，脾脏自然杀伤细胞（ Ｎ Ｋ）、淋巴因子激活的杀伤细胞（ Ｌ Ａ Ｋ）、特异性细胞毒性 Ｔ 淋巴细胞（ Ｃ Ｔ Ｌ）杀伤活性。结果：与荷瘤假照射组相比，７５ ｍ Ｇｙ Ｘ 射线照射组小鼠的上述免疫学参数明显提高（ Ｐ＜ ００５～０１）。结论：低剂量电离辐射可明显增强荷瘤机体的免疫功能，可能具有肿瘤治疗的潜在临床意义。

Stimulatory effect of low dose radiation on the immune function in tumor-bearing mice

Objective:This paper aimes at investigating the effect of whole body irradiation (WBI) with low dose radiation on immune function in tumor bearing mice.Methods:C57BL/6J mine implanted with Lewis lung carcinoma cells in the right thighs were used as an experimental animal model.WBI with 75 mGy X rays was given at the 10 th day after implantation and immunological parameters were detected 18 hours after irradiation.The immunological parameters included the spontaneous incorporation of 3H TdR into thymocytes,the number of splenocytes,the reaction of splenocytes to ConA and LPS,the splenic production of IL 2,the cytotoxic activities of natural killer (NK) and lymphokine activated killer cells (LAK) as well as specific cytotoxic T lymphocytes (CTL).Results:The immunological parameters of irradiated tumor bearing mice were significantly increased compared with those of sham irradiated tumor bearing mice ( P <0 05~0.01).Conclusion:Low dose radiation could significantly increase the immune function of tumor bearing mice,and this stimulatory effect may be of some potential significance in tumor therapy.