五味子淫羊藿混合提取物对东莨菪碱致小鼠学习记忆障碍的改善作用

目的：探讨五味子与淫羊藿混合提取物（MSEE）对东莨菪碱诱导的小鼠学习及记忆障碍的影响，为开发辅助改善记忆功能保健食品提供理论依据。方法：ICR小鼠随机分为空白对照组（灌胃给予双蒸水，腹腔注射生理盐水），模型组（灌胃给予双蒸水，腹腔注射东莨菪碱）和150、300、600 mg·kg^-1MSEE组（灌胃给予MSEE，腹腔注射东莨菪碱）。各组小鼠连续灌胃给药30d后，通过Morris水迷宫实验、避暗实验和跳台实验观察各组小鼠的学习记忆能力。分别应用酶联免疫检测法（ELISA）和比色法检测各组小鼠脑组织中乙酰胆碱（ACh）水平和胆碱酯酶（AChE）活性。结果：避暗实验与跳台实验，与模型组比较，600 mg·kg^-1 MSEE组小鼠潜伏期明显延长（P<0.05），各剂量MSEE组小鼠错误次数均明显减少（P<0.05）。Morris水迷宫实验，与模型组比较，300和600 mg·kg^-1MSEE组小鼠潜伏期明显缩短（P<0.05），且经过平台次数和经过空间有效区次数明显增加（P<0.05）。与模型组比较，各剂量MSEE组小鼠脑组织中ACh水平明显增高（P<0.05或P<0.01），AChE活性明显降低（P<0.05或P<0.01）。结论：MSEE具有改善小鼠学习记忆能力的作用，该作用可能与增加脑内ACh水平有关。

Improvement effect of mixed Schisandra-Epimedium extract on learning and memory disorder induced by scopolamine in mice

Objective: To study the effect of mixed Schisandra-Epimedium extract (MSEE) on the learning and memory disorder induced by scopolamine in the mice,and to provide a theoreitical basis for the development of health food of improving auxiliary memory function.Methods: MSEE was extracted by heating reflux extraction. ICR mice were divided into control group (intragastrical administration of water and intraperitoneal saline injection),model group (intragastrical administration of water and intraperitoneal scopolamine injection) and 150,300,600 mg·kg^-1 MSEE groups(intragastrical administration of MSEE and intraperitoneal scopolamine injection).After continuously intragastric administration of MSEE for 30 d,Morris water maze test,step-through test, and step-down test were respectively carried out to observe the effects of MSEE on the learning and memory abilities of the mice.Enzyme-linked immunosorbent assay (ELISA) and clorimetric detection were respectively used to detect the levels of acetylcholine (ACh) and the activities of acetylcholinesterase (AChE) in the brain tissue of the mice in various groups.Results: The results of step-through test and step-down test showed that the latency of mice in 600 mg·kg^-1 MSEE group was significantly prolonged compared with model group (P<0.05),and the error times of mice in 150,300, and 600 mg·kg^-1 MSEE groups were significantly reduced (P<0.05). In Morris water maze test,the latencies of mice in 300 and 600 mg·kg^-1 MSEE groups were significantly shortened compared with model group(P<0.05),and the times crossing the platform and the effective areas in the space were increased (P<0.05). Compared with model group,the ACh levels in brain tissue of the mice in 150,300, and 600 mg·kg^-1 MSEE groups were significantly increased(P<0.05 or P<0.01) and the AchE activities were significantly decreased(P<0.05 or P<0.01).Conclusion: MSEE can improve the learning and memory abilities of the mice,and its effect may be related to increasing the ACh level.