聚乳酸-聚乙醇酸-聚乙二醇血管支架置入对兔动脉粥样硬化模型血清基质金属蛋白酶2，9的影响

目的：探讨生物可降解聚乳酸-聚乙醇酸-聚乙二醇（PLGA-PEG）血管支架置入对兔动脉粥样硬化模型的血清基质金属蛋白酶2，9(MMP-2、MMP-9)的影响。方法：成年健康白兔30只,经球囊血管内膜剥脱法制成髂动脉动脉粥样硬化动物模型，喂养6周后，随机分为两组，实验组(PLGA-PEG组，n=15)，给予血管支架（PLGA-PEG）置入,对照组(n=15)常规喂养。两组动物在支架置入前、置入后7及30 d分别收集耳缘静脉血1 mL, 以SDS-PAGE Zymography法检测MMP-2、MMP-9）及相应酶原（pro MMP-2和pro MMP-9）。结果：实验组支架置入前所测的MMP-2、MMP-9和proMMP-2（11 568±2 219）、(10 364±1 429和（13 649±1 894)INT·mm²）明显高于对照组（6 128±1 562)、(8 519±2 167）和（8 413±2 156）INT·mm²(P<0.05)；但proMMP-2两组比较，差异无显著性(P>0.05)。实验组支架置入后7 d与置入前相比，MMP-2及MMP-9以及相应酶原虽有增高趋势，但差异无显著性（P>0.05）；支架置入30 d后MMP-2及酶原高于置入前（13 935±2 167）、(15 628±1 739）INT·mm² vs （11 568±2 219）、（13 649±1 894）INT·mm²(P<0.01)，MMP-9及酶原亦增高，但差异无显著性（P>0.05）。结论：血清MMP-2及酶原在动脉硬化动物模型中明显增高,血管支架置入后MMP-2增高。

Effects of polylactic acid-polyglycolic acid-polyethylene stent on matrix metalloproteinase 2, 9 in rabbits with atherosclerosis

Objective To investigate the effects of polylactic acid-polyglycolic acid-polyethylene(PLGA-PEG) stent on matrix metalloproteinase(MMPs) in rabbits with atherosclerosis.Methods 30 adult healthy rabbits were divided randomly into two groups:control group(n=15) and PLGA-PEG group(n=15).Right iliac arteries of rabbits of PLGA-PEG group were injured by balloon denuded endothelization.PLGA-PEG stents were implanted in rabbits in PLGA-PEG group.MMP-2,9 and pro MMP-2,9 levels of PLGA-PEG group were determined before implantation,7 and 30 d later by SDS-PAGE zymography.MMP-2,9 and pro MMP-2,9 levels were determined in control groups.Results The concentrations of MMP-2,9 and pro MMP-2 in PLGAPEG group were much higher than those in control group(11 568±2 219,10 364±1 429,13 649±(1 894 INT·mm~2) vs 6 128±1 562,(8 519±)2 167,8 413±2 156 INT·mm~2,P<0.05).There were no significant differences of pro MMP-9 between two groups (P>0.05).In PLGA-PEG group,the levels of MMP-2,9 and pro MMP-2,9 didn′t elevate significantly 7 d later(P>0.05).However the MMP-2 and pro MMP-2 concentrations were still higher 30 d after implantation(13 935±2 167,15 628±1 739 INT·mm~2 vs 11 568±2 219,13 649±1 894 INT·mm~2,P<0.01).Conclusion The serum levels of MMP-2 and pro MMP-2 increase significantly in rabbits with atherosclerosis.The MMP-2 level also increases after implantation.