氩激光对体外培养牛视网膜色素上皮细胞损伤及其防护的实验研究

目的:观察不同功率激光对体外培养的牛视网膜色素上皮(retinalpigmentepithelium,RPE)细胞的损伤以及地塞米松(Dexamethasone,DXM)对激光损伤的保护作用。方法:采用甲基噻唑基四唑(Methylthiazolyte-trazolium,MTT)方法测定不同功率激光照射对RPE细胞的损伤率。并观察在激光照射前后应用3种浓度的DXM后,0.8W功率的激光对RPE细胞的不同损伤率。结果:激光输出功率越大RPE细胞损伤越重。激光照射前应用DXM可减轻激光对RPE细胞的损伤,而照射后应用则无明显作用。结论:激光对RPE细胞的损伤率与激光输出功率成正相关,激光照射前应用DXM对RPE细胞有保护作用

The study of the cytotoxic effects of argon laser photocoagulation on cattle pigment epithelium cells and the preservative effection of dexamethsone

Objective:Observe the cytotoxic effects of argon laser photocoagulationsa on cattle pigment epithelium cells and the protection effect of dexamethsone (Dex) to the RPE cells. Methods:Different power of argon lasers were used to coagulated confluent RPE cells, then the cell killing rates (CKR) were study by MTT assay. we have observed the killing rate of 0.8 W laser after or before use three different concentration Dex also. Results:Different power of lasers have variable CKRs. The CKRs have a line relationship with the laser powers. The use of Dex before laser coagulation could alleviate the effect of laser, but after laser coagulation had no use.Conclusion:The CKRs of laser has positive correlation with its output power. Using Dex before laser coagulation can protect the RPE cells from being killed partly.