Glypican-3mRNA在人原发性肝细胞癌组织中的表达及意义

目的:探讨Glypican-3mRNA在人肝细胞癌中的表达和意义。方法:收集48例肝细胞癌肿瘤组织,相应39例癌旁组织和31例非肿瘤肝组织,采用RT-PCR检测Glypican-3mRNA的表达。结果:48例肝细胞癌肿瘤组织中有37例(77.1%)检测到Glypican-3mRNA表达,其中过度表达率为62.16%。而在相应的癌旁组织和非肿瘤肝组织中表达缺失。肝细胞癌Glypican-3mRNA表达与其瘤体大小和病理分级之间有显著性差异(P<0.05),而与患者年龄、性别、AFP值、HBsAg及包膜状况之间无显著性差异(P>0.05)。结论:Glypican-3mRNA在人肝细胞癌肿瘤组织中过度表达,可作为诊断肝细胞癌的肿瘤特异性标记物之一。

GLYPICAN-3 mRNA EXPRESSION AND ITS SIGNIFICANCE IN HUMAN HEPATOCELLULAR CARCINOMA

Objective: To investigate glypican-3 mRNA expression and its significance in human hepatocellular carcinoma. Methods: The expressions of glypican-3 mRNA were detected by reverse transcrip-tase polymerase chain reaction (RT-PCR) in 48 cases of hepatocellular carcinoma tissues, 39 para-cancertissues and 31 normal hepatocellular tissues respectively. Results: Overexpression of glypican-3 mRNA was found in 37/48(77.1 %) hepatocellular carcinoma tissues. But glypican-3 mRNA was silence in both para-cancer tissues and normal hepatocellular tissues. Significant relationship was observed between positive expression of glypican-3 mRNA and tumor body size and pathology grading in hepatocellular carcinoma(P<0.05). There were no difference between Glypican-3 mRNA express and patient age, sex, AFP value, HBsAgand integument state(P>0.05). Conclusion: The overexpression of glypican-3 mRNA in hepatocellularcarcinoma may be a new gene diagnosis index of hepatocellular carcinoma.