宫颈癌组织中FBW7和c-Myc的表达及相关性分析

目的：检测F盒/WD40重复域蛋白7（FBW7）和c-Myc在宫颈癌组织中的表达情况及临床意义,并分析两者表达的相关性。方法：收集我院2008年1月～2012年12月病理科存档的宫颈癌和正常宫颈组织标本70例,采用免疫组化SP法分别检测46例宫颈癌及24例正常宫颈组织中FBW7及cMyc的表达情况。结果：FBW7和c-Myc在宫颈癌组织中的阳性表达率分别为30.4%（14/46）和69.6%（32/46）,在正常宫颈组织中的阳性表达率分别为66.7%（16/24）和25.0%（6/24）,两组比较差异有统计学意义（χ2=8.454,P=0.004;χ2=12.622,P=0.000）。FBW7表达与宫颈癌临床分期（P=0.036）和病理学分级（P=0.023）显著相关,c-Myc表达与宫颈癌临床分期（P=0.036）、淋巴结转移（P=0.003）和病理学分级（P=0.003）也显著相关。宫颈癌组织中FBW7与c-Myc蛋白表达呈显著负相关（r=-0.667,χ2=6.694,P=0.010）。结论：宫颈癌组织中FBW7低表达,且与c-Myc表达呈负相关,提示FBW7可能通过调控c-Myc泛素化降解参与肿瘤发生、发展。

Evaluation of FBW7 and c-Myc expression and their correlation analysis in cervical cancer

Objective：To investigate the expression of FBW7and c-Myc and their clinical significance and correlation analysis in cervical cancer tissues.Methods：FBW7and c-Myc expression was detected in 46samples of surgically resected cervical cancer and 24samples of normal cervix tissues using immunohistochemical assay.Results：The positive rate of FBW7and c-Myc in cervical cancer tissues（30.4% and 69.6%）were significantly different from those in the normal cervix tissues（66.7%and 25.0%）（χ2= 8.454,P=0.004;χ2=12.622,P=0.000）.The expression of FBW7was significantly correlated with clinical stages（P=0.036）and pathological grades（P=0.023）,and the expression of c-Myc was significantly correlated with clinical stages（P=0.036）,lymph node metastasis（P=0.003）and pathological grades（P =0.003）.FBW was negatively correlated with c-Myc protein expression in cervical cancer tissues.Conclusion：The low-expression of FBW7is negatively correlated with c-Myc and is associated with poor clinical parameters in cervical cancer.FBW7may promote cancer progression by regulating c-Myc ubiquitination degradation in cervical cancer.