| 1，3-丙二醇发酵液的絮凝预处理研究 |
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| 引用本文： | 张婷婷,张力,王贺新,韩香,刘玮,郝鹤,雷鹏森.1，3-丙二醇发酵液的絮凝预处理研究[J].武警医学院学报,2009,18(5):392-395. |
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| 作者姓名： | 张婷婷 张力 王贺新 韩香 刘玮 郝鹤 雷鹏森 |
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| 作者单位： | 1. 武警医学院化学教研室,天津,300162
2. 武警天津总队医院放射科,天津,300162 |
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| 摘 要： | 【目的】对含1，3-丙二醇的发酵液进行絮凝预处理研究。【方法】实验采用三种阳离子型聚丙烯酰胶（以下简称CPAM）作为絮凝剂去除含1，3-丙二醇发酵液中的菌体和蛋白质大分子杂志。考察不同条件下的絮凝处理对发酵液中1，3-丙二醇和过滤效果的影响。【结果】实验研究得到适宜的1，3-丙二醇的发酵液絮凝条件：采用F04800SH型阳离子型聚丙烯酰胺，絮凝剂用量0．2g／L，pH7．0～11．0，搅拌速度200dmin，温度15℃～60℃，该条件下预处理的发酵液絮凝率大于95％，絮凝率超过95％。【结论】实验研究确定的絮凝预处理不会造成1，3-丙二醇的损失且能加快过滤速度，是一种有效的1，3-丙二醇发酵液预处理方法。
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| 关 键 词： | 1 3-丙二醇 发酵液 预处理 絮凝 |
Flocculation of fermented broth with 1,3-propanediol |
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| ZHANG Ting-ting,ZHANG Li,WANG He-xin,HAN Xiang,LIU Wei,HAO He,LEI Peng-sen.Flocculation of fermented broth with 1,3-propanediol[J].Acta Academiae Medicinae CPAPF,2009,18(5):392-395. |
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| Authors: | ZHANG Ting-ting ZHANG Li WANG He-xin HAN Xiang LIU Wei HAO He LEI Peng-sen |
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| Affiliation: | ZHANG Ting- ting, ZHANG Li, WANG He- xin, HAN Xiang, LIU Wei, HAO He, LEI Peng- sen (Department of Chemistry, Medical College of Chinese People＇ s Armed Police Force, Tanjin 300162, China) |
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| Abstract: | Objective] Study on the flocculatim of fermented broth with 1, 3- Propanediol. Methods] The fermented broth with 1, 3 - Propanediol was pretreated wsing three CPAM. Effects of removal of thalli and protein by flocculation were investigated. The influence of floceulation on the 1, 3 - Propanediol comeentratim and the filtration speed was studied. Results] The optimal floceulation conditions were as follows： using the F04800SH - type CPAM flocculant, flecculant 0.2 g/L, pH7.0-11.0, agitation speed 200 r/win, temperature 15 ℃-60 ℃, and the flocculation ratio was more than 95%. Conclusions] The 1, 3 - propanediol concentration was not changed and the speed of filtration was quickened. The method was proved perfect in the pretreating of fermented Broth with 1, 3 - Propanediol. |
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| Keywords: | 1 3- Propanediol Fermented Broth Pretreatment Flocculation |
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