一种高成活率培养大鼠原代心肌细胞的方法

【目的】探讨原代心肌细胞培养的条件和方法。【方法】取新生1～3d的SD大鼠心室肌，O．25％胰蛋自酶-0．02％EDTA消化后，培养于DMEM—F12培养液中，用差速贴壁法和化学抑制法相结合去除非心肌细胞。倒置显微镜下观察形态学变化，透射电镜观察细胞超微结构。【结果】培养的心肌细胞平均存活率（96．33±6．73）％，培养第4天细胞搏动率达（95．3±9.2）％，并出现单层同步搏动，具有典型的心肌细胞形态特征。【结论】本研究的心肌细胞培养方法操作简便，成活率高，是一种较为理想的原代心肌细胞培养方法。

A high-survival-rate culture method of rat primary myocardial cells

Objective] To study the culture condition and method of primary myocardial cells. Methods] After digested through0.02% EDTA with 0.25% trypsin, ventricular muscles of neonatal SD rats （1-3 d） were cultured in DMEM-F12 culture medium, and then non myocardial cells were removed through combined methods of differential adhesion and chemical inhibition. Morphological changes were observed under inverted microscope, and the ultrastructure was observed by transmission electron microscope. Results ] The average survival rate of cultured myocardial cells was （96.33 ± 6.73）%, and on the fourth day, beating rate of cultured cells was （95.3 ± 9.2）% and monolayer synchronous pulsation emerged, which was the typical morphological features of myocardial cells. Conclusion ] With the advantages of simple operation and high survival rate, the culture method in this study was an ideal culture method of primary myocardial cells.