高效液相色谱法测定不同产地续断药材中木通皂苷D的含量

【目的】考察不同来源续断药材中木通皂苷D的含量，为评价续断药材质量提供参考。【方法】采用反相高效液相色谱法测定续断药材中木通皂苷D的含量，色谱柱KromasilC18柱（250mm×4．6mm，5μm），流动相为乙腈-水梯度洗脱，检测波长212nm，流速为1．0ml／min。【结果】在实验条件下木通皂苷D分离效果良好，0．036～3．695μg范围内其线性关系良好（r=0．9999）；平均加样回收率为104．87％，RSD为1．85％。【结论】该方法准确、可靠，为续断药材的质量评价提供研究基础。

Determination of the contents of akebia saponin D in the root of Dipacus asperoides from various habitats by HPLC

Objective ]To compare the contents of akebia saponin b in the root of Dipacus asperoides from different areas. Methods ] HPLC analytical method was established by using Kromasil C 18 column （250 mm x 4.6 mm, 5μm） with acetonitrile and water （gradient elution） as mobile phase and the detection wavelength was 212nm with a flow rate of 1.0 ml/min. Results] The akebia saponin D in root extract were well separated by this method. The linear range was between 0.036-3.695 μg , r = 0.9999. The average recovery was 104.87% （RSD = 1.85% ）. There was great difference in the contents of akebia saponin D, ranging from 0.46% to 14.48%. Conclusion ]This method is accurate and reliable, laying a basis for the quality assessment of Dipsaeus asperoides.