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| 实验模型下两种全基因组扩增技术对指纹DNA检验的效能 | |
| 引用本文： | 邓建强,刘宝琴,蔡继峰,李文慧,龙仁,侯一平.实验模型下两种全基因组扩增技术对指纹DNA检验的效能[J].中国热带医学,2012,12(5):524-526,561. |
| 作者姓名： | 邓建强 刘宝琴 蔡继峰 李文慧 龙仁 侯一平 |
| 作者单位： | 1. 海南医学院法医鉴定中心,海南海口,570102 2. 西安现代妇产医院,陕西西安,721000 3. 中南大学湘雅医学院法医学系,湖南长沙,410078 4. 四川大学华西基础与法医学院,四川成都,610041 |
| 基金项目： | 国家自然科学基金资助项目，高校博士点专项科研基金资助项目 |
| 摘 要： | 目的对简并寡核苷酸引物PCR（Begenerate oligonucleotide-primed PCR,DOP-PCR）和扩增前引物延伸PCR（Primer extension pre-amplification PCR,PEP-PCR）用于指纹检材DNA检验的价值进行研究。方法建立2种指纹检材DNA模型,分别用普通PCR、DOP技术和PEP技术三种方法对指纹DNA样本进行STR分型,对三种方法的效能进行比较评价。结果实验模型1条件下,三种方法均不能获得满意分型结果;模型2条件下,DOP和PEP两种方法可以增加STR分型的成功率和信息量。结论 DOP和PEP技术必须结合高效的DNA提取技术才能最大程度发挥其检验效能。 |
| 关 键 词： | 法医学 全基因组扩增 指纹DNA 简并寡核苷酸引物 扩增前引物延伸 |
Effects of two whole genome amplification methods for fingerprint samples' genotyping under two experimental model conditions |
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| Affiliation: | DENG Jian-qiang,LIU Bao-qin,CAI Ji-feng,LI Wen-hui,et al.(1.Department of Forensic Medicine,Hainan Medical University,Haikou,570102,Hainan P.R.China) |
| Abstract: | Objective To estimate the practical value of degenerate oligonucleotide-primed PCR（D0P-PCR）and primer extension pre-amplification PCR（PEP-PCR）protocols for fingerprint samples genotyping.Methods DNA samples obtained from fingerprints under two experimental conditions were genotyped by three ways：directly analysis,DOP and PEP protocol.Their effects were compared.Result No satisfied results were received by three methods under model-1 condition,but DOP and PEP protocols improved successful rates and message amounts for fingerprint samples genotyping under experimental model-2 condition.Conclusions In order to perform their powerful capability,DOP and PEP protocol must combine efficient DNA collecting technology. |
| Keywords: | Forensic medicine Whole genome amplification Fingerprint sample Degenerate oligonucleotide-primed PCR Primer extension pre-amplification PCR |
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