猪带绦虫成虫凝溶胶蛋白基因的生物信息学分析

目的：分析猪带绦虫（Taenia solium）成虫凝溶胶蛋白（gelsolin,GEL）基因及编码蛋白的结构和功能。方法：利用生物信息网站美国国家生物技术信息中心（NCBI）和瑞士生物信息学研究所的蛋白分析专家系统（ExPASY）中生物信息学分析工具,并结合其它分析软件,从获得的猪带绦虫成虫全长cDNA质粒文库的表达序列标签（EST）中识别凝溶胶蛋白基因,分析该基因的结构并预测其编码蛋白质的结构和功能特征。结果：猪带绦虫成虫凝溶胶蛋白基因与细粒棘球绦虫凝溶胶蛋白的一致性为88%,相似性为93%;全长1 514 bp,编码区为167~1 263 bp,编码365个氨基酸,无各种亚细胞定位序列,具有多个潜在的磷酸化位点,蛋白在溶液中性质稳定。结论：应用生物信息方法从猪带绦虫成虫cDNA质粒文库中筛选出了猪带绦虫凝溶胶蛋白cDNA全长序列并预测其结构与功能。

Bioinformatics Analysis on Gelsolin Gene of Taenia Solium Imago

Objective: To analyze the structure of gelsolin gene from Taenia solium adults(Ts GEL) and the structure and function of the encoded protein.Methods: Gelsolin gene was identified from T.solium full-length cDNA plasmid libratory by analyzing tools.Its structure was analyzed,and the structure and function characteristics of the encoded protein were predicted.Results: Consistency and similarity of Ts GEL with Acephalocystis granulosus gelsolin gene were 88% and 93% respectively.The full length of Ts GEL was 1 514 bp.Its coding region was 167~1 263,encoding 365 amino acids.The encoded protein didn’t contain any kinds of subcellular localization sequence,but contained several potential phosphorylation sites.It was stable in solution.Conclusions: The full cDNA sequence of gelsolin gene is screened from cDNA plasmid library of T.solium imago with bioinformatical method.The structure and function of the gene and encoded protein are predicted.