抑癌基因FHIT和WWOX在胃腺癌中的表达及其临床意义

目的通过检测FHIT蛋白和WWOX蛋白在胃腺癌组织中表达情况和FHITmRNA和WWOXmRNA在胃腺癌和癌旁正常组织的表达情况,分析两者与临床病理特征之间的关系及两者之间是否具有相关性。方法随机抽取佳木斯大学附属第一医院普外科2000年到2011年期间的70例胃腺癌切除组织标本,采用免疫组化和RT-PCR方法检测胃腺癌组织中临床病理关系及胃腺癌与癌旁正常组织(距肿瘤边缘>5CM)的关系。结果 (1)FHIT和WWOX均阳性表达28例。FHIT和WWOX均阴性表达24例。FHIT阳性而WWOX阴性8例。FHIT阴性而WWOX阳性10例。FHIT和WWOX在胃腺癌中的表达成正相关性。(2)FHITmRNA与WWOXmRNA在70例胃腺癌组织中表达较癌旁组织低者分别有22例和28例;较癌旁胃正常组织高者均有6例。FHITmRNA和WWOXmRNA在胃腺癌的表达低于癌旁胃组织。结论 (1)FHIT和WWOX在胃腺癌组织中阳性表达率较低,在组织学分级,病理分期中呈正相关,而与年龄和病理分型无相关性。(2)FHIT和WWOX的在胃腺癌组织中与癌旁胃正常组织中差异有显著性。(3)FHITmRNA和WWOXmRNA在胃腺癌组织中的低表达呈低度正相关性。(4)FHIT和WWOX是重要的抑癌基因。

The Expression of Tumor Suppressor Genes FHIT and WWOX in Gastric Carcinoma and Its Clinical Significance

Objective Through detection of FHIT and WWOX protein in gastric adenocareinoma tissue expression level and the detection of FHITmRNA and WWOXmRNA in gastric carcinoma and adjacent normal tissue expression, analyzing the relationship between the two and the clinical and pathological characteristics, and whether they have any correlations. Methods Randomly select 70 cases of gastric cancer resected tissue specimen in general surgery from 2000 to 2011 in the first affiliated hospital of Jiamusi University, using immunohistochemical and RT - PCR method for the detec- tion of gastric adenocarcinoma in relation to clinicopathologic, as well as the relationship between gastric carcinoma and adjacent normal tissues （from the tumor margin 〉 5cm）. Results 1. The FHIT and WWOX protein expression is positive ralated in gastric adenocareinoma. 2. In gastric adenocar- cinoma the FHIT and WWOX mRNA expression positive rate was significantly lower than the adjacent normal tissue. Conclusions 1. The positive ex- pression rate of FHIT and WWOX in gastric adenocarcinoma is low, and the histological grade, pathological stage is closely correlated while age and pathologic type have no correlation. 2. FHIT and WWOX were significantly different between gastric adenocarcinoma and gastric tissues of normal. 3. The low expression of FHIT and WWOX in gastric adenocarcinoma tissue are minuent positive related. 4. FHIT and WWOX are both important tumor suppressor genes.