| 87株幽门螺旋杆菌VacA等位基因的分析 |
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| 引用本文： | 王晶桐,刘玉兰.87株幽门螺旋杆菌VacA等位基因的分析[J].中国消化内镜,2008(11):37-42. |
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| 作者姓名： | 王晶桐 刘玉兰 |
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| 作者单位： | 北京大学人民医院消化科; |
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| 摘 要： | 目的以研究方法LiPA（Line Probe Assay）分析VacA等位基因的表达，了解幽门螺旋杆菌致病机理的理解。方法从三个不同城市87位进行胃镜检查患者的胃粘膜中培养出幽门螺旋杆菌，提取DNA，用LiPA方法分析VacA等位基因。结果（1）87位患者以sic（88．5％）和m2a（63．2％）分布为主，未发现s1b和s2；（2）混合菌株感染率为41．4％，远远高于西方国家，其中上海的混合感染率最高（62．5％），与北京（41．0％）和南宁（20．8％）相比具有显著性差异，P〈0．01；（3）北京、海、南京三个不同城市s1、m等位基因亚型分布率存在差异；（4）溃疡病和非溃疡病患者m1和m2的分布率没有显著性差异。结论我国幽门螺旋杆菌多重菌株感染率较高，不同的m基因型与消化性溃疡的发生无显著性相关。
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| 关 键 词： | 幽门螺旋杆菌 VacA等位基因 致病机理 临床分析 |
Analysis of Alleles of VacA of 87 Strains of Helicobacter Pylori |
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| WANG Jing-tong LIU Yu-lan Gastroenterology.Analysis of Alleles of VacA of 87 Strains of Helicobacter Pylori[J].Digestive Disease and Endoscopy,2008(11):37-42. |
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| Authors: | WANG Jing-tong LIU Yu-lan Gastroenterology |
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| Affiliation: | WANG Jing-tong LIU Yu-lan Gastroenterology Department of People\'s Hospital of Peking University Beijing,100044,China |
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| Abstract: | Objective:Our aim is to study the distribution of alleles of vacA and the relationship between alleles of vacA and the presence of peptic ulcer.Methods:Helicobacter pylori were cultured from gastric biopsy of patients who were endoscoped.DNA abstracted from bacteria was analyzed with LiPA after PCR to determine the alleles' type of vacA.Results:1,The prevalence rates of s1c(88.5%)m1(46.0%)and m2a(63.2%)are dominant among the 87 patients;Neither s1b nor s2 were detected.2,Multi-strain infection rate is 41.4%... |
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| Keywords: | Helicobacter pylori VacA alleles Pathogenesis |
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