枸杞多糖对小鼠骨髓树突状细胞成熟的影响

目的：研究枸杞多糖对小鼠骨髓树突状细胞（DC）表型及功能成熟的影响。方法：小鼠骨髓细胞用GM-CSF和IL-4培养5d，然后用免疫磁珠法纯化DC细胞，实验组加入枸杞多糖（100μg／ml），空白对照组加等量RPMI1640，阳性对照组加LPS（100ng／ml），3组分别同时作用48h。流式细胞仪检测细胞表型和细胞摄取抗原的能力，ELISA检测产生的细胞因子IL-12，混合淋巴细胞反应检测细胞提呈抗原的能力。结果：用枸杞多糖刺激的小鼠骨髓DC表达I-A／I-E、CD11c和分泌IL-12能力比空白对照组增加，吞噬FITC-dextran的能力下降，并可促进同种异基因T细胞的增殖。结论：枸杞多糖可以促进体外培养的小鼠骨髓DC的成熟，促进DC诱导的免疫应答启动。

Stimulation by Lycium bararum polysaccharides of the maturation of dendritic cells in murine bone marrow

Objective: To study the effect of Lycium bararum polysaccharides(LBPs) stimulation on the maturation of murine bone marrow derived dendritic cells(BMDCs).Methods: Murine bone marrow cells were cultured in GM-CSF and IL-4 for 5 days,then were purified with a MACS column.Respectively,BMDCs were stimulated with LBPs,LPS and RPMI1640 for 2 days.Cell phenotypes and antigens uptake by BMDCs were analyzed by flow cytometry.Cytokines released by BMDCs were detected.The antigen presenting by BMDCs was evaluated by mixed lymphocyte responses.Results: Compared with to the BMDCs that only subjected to RPMI 1640,the expression of I-A/I-E,CD11c and secretion of IL-12 by BMDCs stimulated with LBPs were increased,the phagocytosis of FITC-dextran by BMDCs stimulated with LBPs was impaired but the activation of proliferation of allogenic lymphocytes by BMDCs was strengthened.Conclusion: LBPs promote not only the maturation of cultured murine BMDCs in vitro,but also the immune response initiation induced by BMDCs.